急性咽结膜炎暴发流行的病毒病原学研究

目的 探讨１９９７年夏深圳地区暴发浒的急性咽结膜炎的病毒病原学。方法 采用急性咽结膜炎患儿急性期眼、咽拭子进行病毒分离，并同时用ＰＣＲ法检查标本中病毒及分离病毒鉴定，对分离出部分毒株采用多个内切酶进行内切酶分析。此外，还测定患儿双份血中病毒的中和抗体增长。结果 １４份患儿咽拭子和１４份眼拭子痍是性率分别为７１．４３％和６４．２９％；ＰＣＲ法检测标本中病毒ＤＮＡ的检出率分别为７８．５７％和７１．４３

Viraletiology study of acute conjunctivitis

Objective To study the viral etiology of acute conjunctivitis broke out in Shenzhen in 1997. Methods Isolated the virus from eye and pharynx swabs of acute conjunctivitis in acute stage ,and detected virus nucleic acid by polymerase chain reaction(PCR). DNA of viral strains were subjected to restriction enzyme analysis using several endonucleases.Also,neutralizing antibody of paired sera were detected.Results The positive virus isolation rates of 14 pharynx and eye swabs were 71 43% and 64 29%,respectively;and that of the virus DNA by PCR were 78 57% and 71 43%, respectively.All of the strains isolated were adenovirus type 3. Three paired viral strains chosen and suabjected to restriction enzyme analysis proved belonging to Ad 3a 2 gene type. The neutralization test of paired sera of 24 cases showed that the antibody titers had 4 fold rising or above.Neutralization test of isolated viruses with paired sera of itself was carried out,the level of neutralizing antibody in convalescent atage was above 4 times as compared with that of acute stage.Conclusion The results showed that outbreak of acute conjunctivitis occured in 1997 in Shenzhen was caused by adenovirus type 3.