内皮型一氧化氮合酶基因G894T多态性与替米沙坦降压效果的相关性

目的探讨内皮型一氧化氮合酶(eNOS)基因G894T多态性与替米沙坦降压效果的相关性。方法轻、中度原发性高血压(以下简称高血压)患者75例服替米沙坦单药4周,观察临床疗效,并采用多聚酶链式反应-限制性内切酶片段长度多态性方法(PCR-RFLP)分析患者eNOS基因G894T的基因型。结果高血压患者eNOS基因G894T3种基因型分别为GG44.0%、GT41.3%、TT14.7%,其中G、T等位基因频率分别为64.7%和35.3%。治疗前GG+GT基因型组和GG基因型组患者的收缩压、舒张压和平均动脉压比较差异均无统计学意义。治疗4周后,GT+TT基因型组患者舒张压下降的幅度〔(6.1±7.3)mmHg(1mmHg=0.133kPa)〕大于GG基因型组〔(4.0±8.0)mmHg〕,但差异无统计学意义(P=0.624),收缩压下降的幅度〔(7.7±12.8)mmHg〕比GG基因型组〔(9.6±19.0)mmHg〕小,差异亦无统计学意义(P=0.623)。结论在本组高血压人群中未发现eNOS基因G894T多态性与血管紧张素Ⅱ受体拮抗剂替米沙坦类药物降压效果的相关性。

Association of G894T Polymorphisms of Endothelial Nitric Oxide Synthase Gene with the Antihypertensive Effect of Telmisartan

Objective To investigate the association of the G894T polymorphism of endothelial nitric oxide synthase(eNOS) gene with the antihypertensive effects of telmisartan in essential hypertensive patients. Methods Seventy-five patients with mild to moderate essential hypertension diagnosed according to criteria of hypertension of WHO/ISH were enrolled in and treated with telmisartan for 4 weeks. Polymerase chain reaction combined with restrictive enzyme digestion was used to detect the G894T polymorphisms of eNOS gene in patients. Results There are three genotype-GG, GT, TT(their genotype frequencies is respectively 44.0%, 41.3%, 14.7%), the frequency of G allele is 64.7% and T allele is 35.3%. Before treatment, There is no statistically significant in SBP or DBP or MAP between GG genotype patients and GT+TT genotype patients; After 4-weeks' treatment of telmisartan, the decrease of DBP with GT+TT genotype patients 〔(6.1±7.3)mmHg (1 mmHg=0.133 kPa)〕was higher than that of GG genotype〔(4.0±8.0)mmHg〕, but it is not statistically significant(P=0.624); however, the decrease of SBP with GG genotype patients 〔(9.6±19.0)mmHg〕was higher than that of GT+TT genotype〔(7.7±12.8)mmHg〕,but it is not statistically significant(P=0.623). Conclusion We can not found that the association of eNOS gene G894T polymorphisms with antihypertensive effects of telmisartan in this selected hypertensive patients.