补阳还五汤及有效组分生物碱和苷对大鼠血小板聚集及血小板环核苷酸的影响

目的探讨补阳还五汤及其有效组分生物碱和苷抗血小板聚集机制。方法大鼠分别给予补阳还五汤原方、生物碱、苷和噻氯匹定，进行ADP诱导的血小板聚集实验。取聚集前、后的血小板提取cAMP、cGMP，采用放免法检测血小板cAMP、cGMP。结果各组血小板聚集比较，生物碱组、苷组和噻氯匹定组血小板聚集强度与空白组相比显著降低（P〈0．01）。原方组血小板聚集强度与空白组相比显著降低（P〈0．05）。血小板聚集后cAMP含量降低（P〈0．01）．而原方、生物碱、苷和噻氯匹定均可抑制ADP诱导的血小板cAMP下降（P〈0．05，P〈0．01）。血小板聚集后cGMP含量降低（P〈0．01），原方、生物碱、苷和噻氯匹定也可抑制聚集后血小板cGMP下降（P〈0．05，P〈0．01）。结论生物碱、苷、原方和噻氯匹定可抑制ADP诱导的大鼠血小板聚集，各药可抑制血小板聚集后血小板内cAMP、cGMP的下降，提示其抗血小板聚集作用是通过抑制聚集后血小板内环核苷酸降低而实现的。

Effect of Buyang huanwu decoction and its active fraction alkaloid and glycoside on platelet aggregation and cyclic nucleotide in rats

Objective To explore the effect of Buyang Huanwu Decoction （BHD） and its active fraction alkaloid and glycoside extracted from BHD on platelet aggregation and its mechanism in rats. Methods The rats were administered with BHI）, alkaloid, glycoside, and ticlopidine respectively and platelet aggregation was induced by ADP. The levels of cyclic adenosine monophosphate （cAMP） and cyclic guanosine monophosphate （cGMP） in the platelet in rats before and after platelet aggregation were measured by radioimmunoassay. Results Potency of platelet aggregation of BHD, alkaloid, glycoside, ticlopidine groups was markedly lower than that of the control group （P〈0.05, P〈0. 01） . The levels of cAMP were decreased after the platelet aggregation （P〈 0. 01）. BHD, alkaloid, glycoside and ticlopidine could inhibit the decrease of cAMP induced by ADP in platelets （P〈0.05, P〈0.01）. The levels of cGMP were decreased after the platelet aggregation （P〈0. 01）. BHD, alkaloid, glycoside and ticlopidine could inhibit the decrease of cGMP in platelets after the aggregation （P〈0. 05, P〈0.01）. Conclusion Alkaloid glycoside, BHD, and ticlopidine could inhibit the platelet aggregation induced by ADP, and inhibit the decrease of cAMP and cGMP after the platelet aggregation. This suggested that its antiplatelet aggregation may be related to inhiting the decrease of cyclic-nucleotide in platelets after the aggregation.