环孢菌素A对大鼠肺纤维化的治疗作用

研究环孢菌素A(CsA)对肺纤维化的治疗作用。方法应用博莱霉素(BLM)复制大鼠肺纤维化模型,观察CsA对支气管肺泡灌洗液(BALF)中细胞成分、肺组织学变化以及应用SABC法检测肺组织中纤维连接蛋白(FN)和血小板衍化生长因子(PDGF)表达的作用。结果应用博莱霉素后第7天即有严重肺泡炎性改变,晚期有严重的肺纤维化,而CsA组,早期只有轻度炎性改变,BALF中细胞计数较BLM组低(P<0.001),FN及PDGF表达显著弱于BLM组(P<0.01)。结论CsA通过抑制FN及PDGF的表达治疗肺纤维化。

EFFECTS OF CYCLOSPORIN AON PULMONARY FIBROSIS IN RATS

Objective:To study the effects of cyclosporin A on pulmonary fibrosis induced by bleomycin in rats.Methods:Forty five wistar rats were divided into three groups in random:Saline,untreated model and cyclosporin A treated group.Bronchoalveolar lavage were performed in these rats for the enumeration of cells.Lung tissues of tested animals were pathologically observed and immunohistochemically stained.Results:Seven days later after using bleomycin instillation,Serious alveolitis presented,and the serious pulmonary fibrosis formed at 28th day.In contrast to the bleomycin group,in the early course of the cyclosporin A treated group,there was mild inflammatory changes observed under microscope.The expressions of FN and PDGF decreased more in the cyclosporin A treated group compared with those of the same stage in the bleomycin group,There was almost disappearance of pulmonary fibrosis in the cyclosporin A treated group at 28th day.Conclusion:Cyclosporin A may have an inhibitory effect on the process of the pulmonary fibrosis.