西妥昔单抗单用及与化疗药物联合对膀胱癌细胞株T-24的增殖抑制作用

目的 观察西妥昔单抗(Cetuximab)单用及与丝裂霉素(MMC)、阿霉素(ADM)、羟基喜树碱(HCPT)联用对人膀胱癌T-24细胞的增殖抑制作用.方法 选用Cetuximab(0～500 mg/L)和MMC(0～50 mg/L)、ADM(0～10 mg/L)、HCPF(0～10 mg/L),单独或联合作用于T-24细胞,CCK-8法检测细胞增殖抑制作用;金氏公式判断两药联用的协同效应;流式细胞仪检测凋亡率.结果 Cetuximab、MMC、ADM、HCPT对T-24细胞存在程度不同的增殖抑制作用,其20%～30%抑制的工作浓度(IC_(20～30))分别为20.0、0.5、0.1、0.2 mg/L;以该浓度的Cetuximab分别与MMC、ADM、HCPT联合,协同指数q值分别为1.22、1.17、1.25;联合组凋亡率分别为(18.9±1.9)%、(20.1 ±1.9)%、(21.6±2.4)%,显著高于单独药物组(P＜0.05).结论 Cetuximab对膀胱癌T-24细胞存在一定的增殖抑制作用,与化疗药物MMC、ADM、HCPT联合均可取得较好的协同效应,这可能与其进一步促进T-24细胞凋亡有关.

The proliferation inhibition of Cetuximab or combined with chemotherapeutics on human bladder cancer cell line T-24

Objective To investigate the proliferation inhibition of Cetuximab or combined with chemotherapeutics on human bladder cancer cell line T-24. Methods Increasing concentrations of cetux-imab (0-500 mg/L) and chemotherapeutics including M MC (0-50 mg/L), ADM (0-10 mg/L), HCPT (0-10 mg/L) alone or in combination were administrated to human bladder cancer cell line T-24 for 72 h. CCK-8 assay was used to detect the inhibitory effect and King's Formula was used to determine the syner-gistic effect. Flow cytometry technique was also applied to find out the influence of IC_(20-30) concentration of cetuximab or combined with of IC_(20-30) concentration of chemotherapeuties on the cells apoptosis. Results The differently inhibitory effects of cetuximab and chemotherapeutics were observed. The IC_(20-30) concentra-tions of Cetuximab and MMC, ADM, HCPT at 20.0,0.5,0. 1,0.2 mg/L respectively were obtained and subsequently used in the following experiments. The combination index(q) was 1.22,1.17,1.25 and the apeptosis rate of 72 h was (18.9 ± 1.9) %, (20. 1 ± 1.9) % , (21.6 ± 2.4) % respectively (P ＜ 0.05, compared with single cetuximab or chemotherapeutics group). Conclusion Cetuximab combined with chemotherapentics synergistically increases the inhibitory effect on T-24 cells, possibly through the further induction of apoptosis.