维A酸CD437和阿维A抑制人黑素瘤A375细胞的比较

目的探讨合成的维A酸CD437与阿维A对体外培养的人黑素瘤A375细胞的增殖抑制、凋亡诱导、周期阻滞作用及对Bax/bcl-2蛋白表达的影响。方法MTT法测定CD437及阿维A对人黑素瘤A375细胞的增殖抑制作用;流式细胞术检测两种药物诱导细胞凋亡及周期阻滞;细胞爬片SABC免疫细胞化学分析两种药物对细胞Bax/bcl-2蛋白表达的影响。结果干预24h后,10-5mol/L维A酸CD437和阿维A对人黑素瘤A375细胞的增殖抑制率(PIR)和凋亡率分别为58.6%和43.25%,28.03%和17.13%(P<0.05),CD437促A375细胞G0/G1期阻滞,而阿维A无此作用;两药均上调A375细胞Bax蛋白表达和下调bcl-2蛋白表达,但CD437组与阿维A组差异无显著性。结论与阿维A相比较,CD437更有效抑制人黑素瘤A375细胞的增殖及更明显的凋亡诱导和G0/G1期阻滞作用;在辅助治疗黑素瘤方面,CD437比阿维A可能更具潜力。

Comparison of Synthetic Retinoid CD437 anti Acitretin Inhibiting Melanoma A375 Cell

Objective To investigate the effects of synthetic retinoid CD437 and Acitretin on cell proliferation,apoptosis,cycle arrest and Bax/Bcl-2 protein expression of human melanoma A375 cell.Methods MTT assay was used to determine the anti-proliferative effects of CD437 and acitretin on melanoma A375 cell.Flow cytometry was performed to investigate the influence of CD437 and acitretin on cell cycle and cell apoptosis.SABC immunocytochemistry was employed for detection of Bax/bcl-2 protein expressions.Results 10-5 mol/L CD437 was more effective than acitretin in inhibiting proliferation and inducing apoptosis of A375 cell after 24 h treatment,proliferation inhibiting ratio(PIR)and apoptosis ratio(58.6%vs43.25% and 28.03%vs17.13% P<0.05),respectively.CD437 promoted G0/G1 arrest on melanoma A375 cell,however,acitretin could not.CD437 and acitretin could up-regulate the expression of Bax and down-regulate the expression of bcl-2.Conclusion:CD437 is more effective than acitretin in inhibiting proliferation and inducing apoptosis and cycle arrest in human melanoma A375 cell.CD437 may have more potentialities than acitretin for subsidiary treatment of melanoma.