吲哚-3-甲醇对人肝癌细胞株SMMC-7721增殖和凋亡的影响

目的 探讨吲哚-3-甲醇(13C)对人肝癌细胞株SMMC-7721的增殖和凋亡的影响.方法 不同浓度的I3C(100、150、200、250、300、350 μmol/L)处理细胞48 h后,显微镜下观察细胞生长状况；采用WST-1法检测细胞增殖情况,Hoehest33258染色、TUNEL染色检测细胞凋亡情况,Western blot法检测凋亡相关蛋白.结果 I3C能够抑制SMMC-7721细胞增殖,诱导细胞凋亡,处理浓度在250 μmol/L以下时,细胞生长变慢；处理浓度达到300μmol/L时,大量细胞凋亡.I3C浓度高于200 μmol/L时CytC、Cleaved caspase-9和Cleaved caspase-3蛋白表达明显增加,且随着I3C浓度的增加三种蛋白的表达也明显增加.结论 I3C在体外实验条件下可抑制人肝癌细胞株SMMC-7721增殖并诱导其发生凋亡.

Effects of indole-3-carbinol on the proliferation and apoptosis of human liver cancer cell line SMMC-7721

Objective To investigate the effects of indole-3-carbinol on the proliferation and apoptosis of human liver cancer cell line SMMC-7721.Methods The growth status of SMMC-7721 was observed under Microscope.The inhibition of cell proliferation was assayed by WST-1 agent,and the apoptosis was shown with Hochest33258 and TUNEL staining.The apoptosis related protein were detected by Western blot.Results I3C could inhibit the proliferation of SMMC-7721 and induced the apoptosis in a dose-dependent manner.The cell proliferation was slow at doses below 250 μmol/L of I3C.And a large proportion of apoptotic cells were observed at doses above 300 μmol/L of I3C.Furthermore,the expressionsof Cyt C、 Cleaved caspase-9 and Cleaved caspase-3 increased significantly when I3C at concentrations higher than 200 μmol/L.With the increase of I3C concentration,three kinds of protein expression also increased significantly.Conclusion I3C could significantly inhibit the human liver cancer cell proliferation and induce apoptosis in a dose dependent manner.