HPLC 法同时测定宣木瓜中绿原酸、芦丁、金丝桃苷、槲皮素的含量

目的建立宣木瓜药材中绿原酸、芦丁、金丝桃苷、槲皮素的含量测定方法。方法采用HPLC法,Phenomenex luna C18色谱柱(250 mm×4.6 mm,5μm),流动相为0.6%磷酸溶液(A)和乙腈(B),梯度洗脱,检测波长为360 nm,流速设为1.0 ml.min-1。结果绿原酸、芦丁、金丝桃苷、槲皮素线性范围分别为0.035～0.56 g·L-1(r=0.997 8),0.003 2～0.051 2 g·L-1(r=0.999 3),0.004 25～0.068 g·L-1(r=0.999 7),0.003 5～0.056 g·L-1(r=0.999 7),平均加样回收率分别为102.9%(RSD=1.04%)、99.3%(RSD=2.28%)和99.2%(RSD=2.33%)、99.5%(RSD=2.52%)。结论该方法操作简单,结果准确,具有较好的重复性和稳定性,可用于宣木瓜药材的质量控制。

Simultaneous determination of chlorogenic acid,rutin,hyperoside and quercetin in Fructus Chaenomelis by HPLC

Objective To establish an HPLC method for the simultaneous determination of chlorogenic acid,rutin,hyperoside and quercetin in Fructus Chaenomelis.Methods Ahigh-performance liquid chromatography equipped a Phenomenex luna C18（250 mm×4.6 mm,5 μm） column with UV detection used.The mobile phase consisited of 0.6% aqueous phosphoric acid（A） and acetonitrile（B） with gradient elution.The detection wavelength was set at 360 nm and the flow rate was 1.0 ml·min-1.Results The linear ranges of chlorogenic acid,rutin,hyperoside and quercetin were 0.035~0.56 g·L-1（r=0.997 8）,0.003 2~0.051 2 g·L-1（r=0.999 3）,0.004 25~0.068 g·L-1（r=0.999 7）,0.003 5~0.056 g·L-1（r=0.999 7）,respectively;the average recoveries were 102.9%（RSD=1.04%）,99.3%（RSD=2.28%）,99.2%（RSD=2.33%）,99.5%（RSD=2.52%）.Conclusion The proposed method is simple,accurate,and repeatable.which can be used for quality control of Fructus Chaenomelis.