缺血后处理对大鼠肾缺血再灌注损伤的作用

目的：成功建立肾脏缺血后再灌注损伤大鼠模型,研究缺血后处理对肾脏的保护作用。方法：夹闭大鼠左肾动静脉复制大鼠肾缺血再灌注损伤模型。雄性Wistar大鼠30只随机分成3组：缺血再灌注组（I/R,n=10）,缺血后处理组（IPo,n=10）,假手术组（S,n=10）。检测血肌酐浓度（Cr）、尿素氮（BUN）,检测肾组织中超氧化物歧化酶（SOD）活性;取肾组织做HE染色,光镜下观察肾组织病理学变化。结果：S组血清肌酐水平为（63.83±17.26）μmol/L,血清尿素氮水平为（11.56±2.75）mmol/L。I/R组分别达到（175.94±64.53）μmol/L、（42.85±14.67）mmol/L,与S组相比差异有统计学意义（P〈0.05）,IPo组血清肌酐及尿素氮水平分别为（91.51±35.67）μmol/L、（15.91±4.12）mmol/L,与I/R组比较差异有统计学意义（P〈0.05）,而与S组比较差异无统计学意义（P〉0.05）;S组肾组织中SOD含量为（91.3±2.9）U/mgport,I/R组为（55.3±1.6）U/mg-port,与S组比较差异有统计学意义（P〈0.05）,IPo组SOD含量为（71.6±2.7）U/mgport,SOD活性较I/R组升高,差异有统计学意义（P〈0.05）;S组肾小球、肾小管未发现明显的形态学改变,I/R组病理形态与S组相比有显著差异,IPo组病理形态较I/R组明显减轻,统计学上有显著性差异。结论：缺血后处理对肾起保护作用,机制与增强了肾的抗氧化能力,减轻炎性细胞浸润有关。

Effect of ischemic postconditioning on the renal ischemia-referfusionreperfusion injury in rats

Objective：To establish a model of renal ischemia-reperfusion in rats.To investigate the effect of ischemic postconditioning on the renal ischemia-reperfusion（I/R） injury and its mechanism.Method：The model of rat＇s RIRI was established,30 wistar rats were randomly divided into 3 groups：false operating group（S）,ischemia reperfusion group（I/R） and ischemic postconditioning（IPo）.Blood urea nitrogen（BUN）,creatinine（Cr）,superoxide dismutase（SOD） werewas ditecteddetected in each groups.and renal histopathology lesions were examined.Result：In I/R group,BUN and SCr serum levels increased significantly after ischemia reperfusion compared with false operating group（P0.05）,while BUN and SCr serum levels of IPo group decreased significantly after ischemic postconditioning compared with I/R group,there was statistic significance（P0.05）.in I/R group,SOD serum levels decreased significantly after ischemia reperfusion compared with false operating group（P0.05）,while SOD serum levels of IPo group increased significantly after ischemic postconditioning compared with I/R group,there was statistic significance（P0.05）.in S group,gomeruli and tubules have no obvious pathological changes,in I/R group,there are significant distinction of the pathological scale which compared with S group,in IPo group,the histologic damage induced by I/R were significantly ameliorated,there were statistic significance（P0.05）.Conclusion：Ischemic postconditioning play an important protective role in the renal ischemia-reperfusion injury,the mechanism is that which enhance kidney antioxidant capacity and reduce infiltration of inflammatory cells.