LncRNA P5848表达对食管鳞癌细胞生物学行为影响

Effect of lncRNA P5848 on biological behavior of esophageal squamous cell carcinoma

OBJECTIVE Recent studies have shown that long non-coding RNA(lncRNA)is closely related with the occurrence and development of esophageal squamous cell carcinoma(ESCC),which provides new target for the exploration of the diagnosis and treatment.The present study is aimed to examine the expression of lncRNA P5848 in ESCC tissues and cells,and explore the effect and mechanism of lncRNAP5848 on cell proliferation.METHODS Firstly,the expression of P5848 in 16 cases of ESCC(ESCC tissues and adjacent normal esophageal tissues),5 cases of esophageal biopsy mucosal epithelial tissues(normal control group),human esophageal epithelial cell line HEEC and esophageal squamous cell line EC-109,EC-9706 and KYSE-150 were detected by real-time quantitative PCR(qRT-PCR).The over-expression of P5848 in ESCC cell lines was regulated by plasmid transfection.Meanwhile,the cell proliferation rate was detected by MTT and SRB methods.The change of cell cycle was detected by propidium iodide cell cycle detection kit,and apoptosis was detected by caspase-3 activity kit.The change of expression in Bcl-2,p53 and caspase-3 mRNA and protein were detected by qRT-PCR and Western blot.RESULTS The expression of P5848 in ESCC tissues was 0.567(0.483,0.667),significantly lower than that in adjacent tissues[0.975(0.529,1.317)]and normal tissues[1.007(0.772,1.150)],and there was significant difference(Z=-9.688,P=0.011;Z=-13.4,P=0.015).Compared with HEEC cells(1.054±0.200),the expression of P5848 in EC-109,EC-9706,and KYSE-150 cells was 0.562±0.056,0.509±0.095 and 0.629±0.118 respectively,and there was significant difference(F=4.605,P=0.005;F=5.111,P=0.002;F=3.963,P=0.007).Overexpression of P5848 by plasmid transfection significantly upregulated the expression of P5848 mRNA in ESCC,and its overexpression significantly inhibited the proliferation status of esophageal cancer cells(Fvalue was 9.546,13.260,7.394 and3.838,9.705,4.678 respectively,P<0.01).The S-phase cell ratio was reduced(Fvalue was4.673,3.189,4.146;P<0.05),while the G1 phase cell ratio increased(Fvalue was 8.217,6.944,5.074;P<0.01),which suggested that P5848 may inhibit the proliferation of esophageal cancer cells by inhibiting the cells from transitioning from G1 to S phase.In addition,P5848 overexpression can significantly increase apoptosis(Bcl-2 mRNA and protein expression decreasing,Caspase-3 and p53 mRNA and protein expression increasing).CONCLUSIONS Overexpression of P5848 can lead to the change of biological behavior of ESCC,inhibit its proliferation and increase its apoptosis.Therefore,it plays a role in inhibiting the occurrence and development of tumors to a certain extent.