| DMT-1在介导低密度脂蛋白氧化中的作用 |
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| 引用本文： | 简清梅,刘金萍,龚杰,武军驻. DMT-1在介导低密度脂蛋白氧化中的作用[J]. 免疫学杂志, 2007, 23(5): 563-565 |
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| 作者姓名： | 简清梅 刘金萍 龚杰 武军驻 |
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| 作者单位： | 武汉大学基础医学院生物化学与分子生物学系,武汉,430071;荆楚理工学院医学院基础部生化教研室,湖北,荆门,448000;武汉大学基础医学院生物化学与分子生物学系,武汉,430071 |
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| 基金项目： | 教育部留学回国人员科研启动基金 , 荆门市医药产学研基地平台建设项目 |
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| 摘 要： | 目的 通过基因芯片分析发现人单核细胞THP-1氧化LDL的过程中二价金属转运蛋白1(Divalent metal transporter 1,DMT1)的mRNA表达水平显著升高.本实验通过RT-PCR和Western Blot来验证基因芯片的结果.方法用空白,LPS,LDL分别处理THP-1细胞,应用RT-PCR和Western Blot比较在LDL和LPS处理后THP-1细胞DMT-1的表达情况.结果 RT-PCR和Western Blot结果显示,在THP-1氧化LDL的过程中DMT1的表达显著升高.LDL和LPS相比,用LDL处理的THP-1细胞的DMT1的表达增加较LPS处理的明显.结论 RT-PCR和western blot证明了基因芯片的结果,在THP-1氧化LDL的过程中DMT1的表达显著升高,为临床心血管疾病的抗氧化治疗提供新的思路.
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| 关 键 词： | 二价金属转运蛋白1 低密度脂蛋白 动脉粥样硬化 |
| 文章编号： | 1000-8861（2007）05-0563-03 |
| 修稿时间： | 2006-12-262007-03-28 |
Effects of DMT-1 in LDL oxidation mediated by cells |
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| JIAN Qing-mei,LIU Jin-ping,GONG Jie,WU Jun-zhu. Effects of DMT-1 in LDL oxidation mediated by cells[J]. Immunological Journal, 2007, 23(5): 563-565 |
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| Authors: | JIAN Qing-mei LIU Jin-ping GONG Jie WU Jun-zhu |
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| Affiliation: | Department of Molecular Biology, College of Basic Medicine, Wuhan lniversity , Wuhan 430071, China |
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| Abstract: | Objective To confirm the cDNA army analysis results that the DMT1 mRNA level expression was up regulated significantly during the process of LDL oxidation mediated by human THP-1 monocytes by RT-PCR and Western blotting. Methods RT-PCR and Western blotting were used to detect the DMT-lmRNA and DMT-1 protein expressions. Results The results of RT-PCR and Western blotting shown that the expressions of DMT-lmRNA and DMT-1 proteins in LDL oxidation were higher than those of LPS control. Conclusion RT-PCR and Western blotting has confirmed the result of eDNA army. DMT-1 is strongly expressed in LDL oxidation, which provides a new insight for antioxidized treatment of the clinical cardiovascular disease. |
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| Keywords: | DMT1 Low density lipoprotein THP-1 |
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