2型糖尿病患者外周血内皮祖细胞数量和功能的变化

目的观察2型糖尿病（DM）患者外周血内皮祖细胞（EPCs）数量和功能的改变。方法选择2型DM患者16例和对照组19例，密度梯度离心法收集外周血单个核细胞（MNCs），诱导分化培养7d后，荧光染色和流式细胞术分别鉴定贴壁细胞为EPCs。采用二苯基四氮唑嗅盐（MTT）比色法、黏附能力测定实验和体外血管生成实验检测EPCs的增殖能力、黏附能力和体外血管生成能力。结果2型DM患者外周血EPCs数量明显减少[（3．1±1．2）×10^5]：[（3．9±1．1）×10^5]，P〈0．05。且2型DM患者外周血EPCs黏附能力[（50±15）：（60±11）细膨×200视野，P〈0．05]，增殖能力[（0．170±0．056）：（0．225±0．071）OD值，P〈0．05]，体外血管生成能力均明显受损。结论2型DM患者外周血EPCs的数量减少，且其增殖、黏附和血管生成能力受损。

Changes in the Number and Activity of Endothelial Progenitor Cells from Peripheral Blood in Patients with Type 2 Diabetes Mellitus

Objective To investigate the changes in the number and activity of endothelial progenitor cells（EPCs） from peripheral blood in patients with type 2 diabetes mellitus. Methods Choice type 2 diabetes mellitus patients （ n = 16） and control subjects（ n = 19）, peripheral blood mononuclear cells（MNCs） were is isolated by density gradient centrifugation. After 7 days induced differentiation, identified the adherent cells were EPCs by fluorescein staining and flow cytometry. EPCs proliferation, adhesion and in vitro vasculogenesis activity were assayed by MTI＇ chromatometry, adhesion activity assay essay and in vitro vasculogenesis kit respectively. Results The number of EPCs from peripheral blood were significantly reduced in patients with type 2 diabetes mellitus[ （3.1 ± 1.2）× 10^5 ] vs[ （3.9 ±1.1 ） × 10^5 ] ,P 〈 0.05. In addition, the proliferation[ （0. 170 ±0.056 ）vs（0.225±0.071）OD valve,P 〈0.05], adhesion[ （50 ± 15）vs（60 ± 11 ） cells/x 200fields, P 〈 0.05 ] and in vitro vasculogenesis activity of EPCs in patients with type 2 diabetes mellitus were impaired respectively. Conclusion Endothelial Progenitor Cells from peripheral blood in patients with type 2 diabetes mellitus exhibit reduced numbers and impaired proliferation,adhesion and in vitro vasculogenesis activity.