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| 环磷酰胺化疗导致小鼠卵巢功能损伤模型的建立 | |
| 引用本文： | 王方圆,谢超,程蔚蔚,韩伟,赖东梅. 环磷酰胺化疗导致小鼠卵巢功能损伤模型的建立[J]. 上海交通大学学报(医学版), 2011, 31(10): 1398-1402,1408. DOI: 10.3969/j.issn.1674-8115.2011.10.009 |
| 作者姓名： | 王方圆 谢超 程蔚蔚 韩伟 赖东梅 |
| 作者单位： | 1. 上海交通大学医学院附属国际和平妇幼保健院中心实验室,上海,200030 2. 上海交通大学农业与生物学院上海市兽医生物技术重点实验室,上海,200240 3. 上海交通大学药学院再生组学研究室,上海,200240 |
| 基金项目： | 上海交通大学医学院附属国际和平妇幼保健院院内基金(3030448);上海交通大学医工合作面上项目(YG2010MS66)~~ |
| 摘 要： | 目的探索建立化疗后导致小鼠卵巢功能损伤模型的方法。方法以C57BL/6雌性小鼠为研究对象,以75 mg/kg的剂量一次性腹腔注射环磷酰胺,观察小鼠一般情况、动情周期、血清雌激素(雌二醇)水平以及卵巢组织的形态学改变、各级卵泡数变化、抗苗勒管激素分泌情况和组织纤维化程度。以未注射环磷酰胺小鼠作为对照。结果与对照组比较,环磷酰胺注射小鼠血清雌二醇水平降低,卵巢组织抗苗勒管激素分泌减少,卵巢组织间质增生、纤维化明显,各级卵泡数目减少。结论 75 mg/kg的剂量一次性腹腔注射环磷酰胺后可引起小鼠明显的卵巢功能损伤,小鼠生殖内分泌和卵巢组织学的变化与临床上化疗导致卵巢功能损伤患者的情况相似。该方法简便易行、造模时间短、成功率高、死亡率低,是建立化疗导致卵巢功能损伤动物模型的较好方法。 |
| 关 键 词： | 卵巢功能损伤 化疗 动物模型 小鼠 |
Establishment of mouse model of cyclophosphamide-induced ovarian failure |
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| WANG Fang-yuan,XIE Chao,CHENG Wei-wei,HAN Wei,LAI Dong-mei. Establishment of mouse model of cyclophosphamide-induced ovarian failure[J]. Journal of Shanghai Jiaotong University:Medical Science, 2011, 31(10): 1398-1402,1408. DOI: 10.3969/j.issn.1674-8115.2011.10.009 | |
| Authors: | WANG Fang-yuan XIE Chao CHENG Wei-wei HAN Wei LAI Dong-mei |
| Affiliation: | WANG Fang-yuan1,XIE Chao2,CHENG Wei-wei1,HAN Wei3,LAI Dong-mei1(1.Central Research Laboratory,the International Peace Maternity and Child Health Hospital,Shanghai Jiaotong University School of Medicine,Shanghai200030,China,2.Shanghai Municipal Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai200240,3.Laboratory of Regeneration Medicine,School of Pharmacy,China) |
| Abstract: | Objective To establish the mouse model of chemotherapy-induced ovarian failure. MethodsFemale C57BL/6 mice were intraperitoneally injected with cyclophosphamide at the dose of 75 mg/kg.The general conditions,estrous cycles,serum estrogen(estradiol) levels,morphology of ovarian tissues,changes of numbers of follicles,secretion of anti-Mullerian hormone and tissue fibrosis of mice were observed.Mice without cyclophosphamide injection were served as controls. ResultsCompared with controls,the serum estrogen le... |
| Keywords: | ovarian failure chemotherapy animal model mouse |
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