| 枸杞多糖对K562白血病细胞抑制作用及凋亡的研究 |
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| 引用本文： | 崔涛,李梅君,赵艳春.枸杞多糖对K562白血病细胞抑制作用及凋亡的研究[J].辽宁医学院学报,2006,27(1):30-34. |
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| 作者姓名： | 崔涛 李梅君 赵艳春 |
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| 作者单位： | 1. 锦州医学院附属第一医院血液内科,辽宁,锦州,121001
2. 潍坊市昌乐朱刘医院,山东,潍坊,262400 |
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| 摘 要： | 目的 探讨枸杞多糖（LBP-X）诱导人类慢性髓系白血病K562细胞株凋亡。方法 用吖啶橙（AO）荧光染色观察细胞结构的变化，用MTT、比色法测定抑制率，琼脂糖凝胶电泳和流式细胞仪检测法分析细胞凋亡。结果 LBP-X可明显抑制K562细胞的生长，凋亡率呈时间和剂量依赖性。LBP-X作用48h后，荧光显微镜下可见细胞核不规则，浓缩成大小不等的团快，核碎裂呈新月形改变，琼脂糖凝胶电泳可见DNA梯带。流式细胞仪分析图上可见明显的凋亡。结论 LBP-X能诱导K562细胞的凋亡，并呈一定的时间浓度依赖关系。
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| 关 键 词： | 枸杞多糖 K562细胞 凋亡 |
| 文章编号： | 1000-5161（2006）01-0030-05 |
| 收稿时间： | 2005-10-20 |
| 修稿时间： | 2005-10-20 |
Lycium Barbarum Polysaccharide Induces Apoptosis in Chronic Myeloid Leukemia K562 Cells |
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| CUI Tao,LI Mei-jun,ZHAO Yan-chun.Lycium Barbarum Polysaccharide Induces Apoptosis in Chronic Myeloid Leukemia K562 Cells[J].Journal of Liaoning Medical University (LNMU) Bimonthly,2006,27(1):30-34. |
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| Authors: | CUI Tao LI Mei-jun ZHAO Yan-chun |
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| Institution: | 1.Blood Department, the First Affiliated Hospital, Jinzhou Medical College, Jinzhou 121001 China; 2. Zhuliu Hospital, Weifang 262400 China |
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| Abstract: | Objective To study the effect of lycium bararum polysaccharide(LBP-X) on apoptosis of human chronic myeloid leukemia K562 cells.Methods Morphological changes were observed by AO fluorescent staining.Inhibition rate of cell proliferation were stuidied by MTT assay.Apoptosis was analyzed by agarose gel electrophoresis and flow cytometry.Results The growth of K562 cells were inhibited remarkably.Apoptosis of K562 cells depended on dose and time.LBP induced the nuckei of K562 with anomalistic,condensed and fragmentized for 8 hours.Agarose gel electrophoresis of DNA revealed DNA ladder,K562 cells exposed to LBP-X showed apoptotic peaks.Conclosions LBP-X can induce apoptosis of K562 cells,and the inhibition of cell character depended on dose and time. |
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| Keywords: | lycium bararum polysaccharide K562 cell apoptosis |
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