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| 光镜和电镜研究免疫酶的组织化学染色法 | |
| 引用本文： | 高俊峰 名仓宏. 光镜和电镜研究免疫酶的组织化学染色法[J]. 中国医科大学学报, 1990, 19(1): 29-32 |
| 作者姓名： | 高俊峰 名仓宏 |
| 作者单位： | 中国医科大学第二电子显微镜室(高俊峰)，名古屋大学医学部生体防御研究室(名仓宏) |
| 摘 要： | 本文对免疫酶组织化学的样品制备程序和染色方法做了详细的阐述。用直接法、间进法和ABC法,对IgA、Leu4、HLA-DR、CEA和4型角蛋白的定位,进行了光镜和电镜的观察,染色阳性反应显著,获得了满意的效果。并对染色技巧做了分析和探讨。 |
| 关 键 词： | 免疫组织化学 辣根过氧化物酶 二氨基联苯胺 过氧化物酶抗过氧化物酶 卵白素生物素抗过氧化物酶复合物 |
IM AND EM STUDIES OF HTSTOCHEMICAL STAINING METHOD FOR THE IMMUNOENZYME |
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| Gao Junfeng,et al The Second Electronic Microscopic Laboratory. IM AND EM STUDIES OF HTSTOCHEMICAL STAINING METHOD FOR THE IMMUNOENZYME[J]. Journal of China Medical University, 1990, 19(1): 29-32 | |
| Authors: | Gao Junfeng et al The Second Electronic Microscopic Laboratory |
| Abstract: | In this article, the precedures for histochemical sample preparation ofimmunoenzyme and its staining method are elaborated. With direct, indirectand ABC methods, the local distribution of IgA, Leu4, HLA-DR, CEA andtype 4 keratin in human intestinal mucosa was observed under LM and EM.The positive reaction of staining was remarkable with satisfactory results. The skill of staining is also analysed and discussed. |
| Keywords: | immunohistochemistry horseradish peroxidace diaminobenzidine peroxidase-anti-peroxidase avidin-biotin-peroxidase complex |
| 本文献已被 CNKI 维普 等数据库收录！ | |