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| 高效液相色谱法测定大鼠血浆中吴茱萸次碱浓度 | |
| 引用本文： | 侯晓虹,于治国,杜惠莲,艾莉,裴建梅,毕开顺. 高效液相色谱法测定大鼠血浆中吴茱萸次碱浓度[J]. 沈阳药科大学学报, 2001, 18(5): 335-337 |
| 作者姓名： | 侯晓虹 于治国 杜惠莲 艾莉 裴建梅 毕开顺 |
| 作者单位： | 1. 沈阳药科大学药学院 2. 沈阳医学院 附属卫生学校 3. 辽宁省医药工业研究院 |
| 摘 要： | 建立大鼠血浆中吴茱萸次碱的反相高效液相色谱法。以乙腈 水 (5 8∶4 2 )为流动相 ,地西泮为内标 ,在波长 3 4 5nm处检测。吴茱萸次碱浓度在 0 0 2～ 1 6μg/mL呈线性 ,相关系数为 0 999,高中低 3种浓度平均萃取回收率均大于 80 0 % |
| 关 键 词： | 反相高效液相色谱法 吴茱萸次碱 大鼠血浆 |
| 文章编号： | 1006-2858(2001)05-0335-03 |
| 修稿时间： | 2001-02-20 |
Determination of the rutaecarpine in the rat plasma by HPLC |
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| HOU Xiao hong ,YU Zhi guo ,DU Hui lian ,AI Li ,PEI Jian mei ,BI Kai shun. Determination of the rutaecarpine in the rat plasma by HPLC[J]. Journal of Shenyang Pharmaceutical University, 2001, 18(5): 335-337 | |
| Authors: | HOU Xiao hong YU Zhi guo DU Hui lian AI Li PEI Jian mei BI Kai shun |
| Affiliation: | HOU Xiao hong 1,YU Zhi guo 1,DU Hui lian 2,AI Li 3,PEI Jian mei 1,BI Kai shun 1 |
| Abstract: | An accurate, convenient and sensitive HPLC method with UV detection was established to determine the rutaecarpine in the rat plasma. The rutaecarpine was determined by RP HPLC.The pretreatment of the plasma sample was based on single liquid liquid extraction. Chromatographic conditions included mobile phase acetonitrile water(58∶42), internal standard diazepam, detector UV, 345 nm . The working curve was linear in the concentration range of 0 02～ 1 6 μg/mL . The average recoveries of high,mid and low concentrations of rutaecarpine were higher than 80 0%. This method was used for determination of the concentration of rutaecarpine in the rat plasma. It is satisfactory for the pharmocokinetics study of rutaecarpine. |
| Keywords: | HPLC rutaecarpine rat plasma |
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