外阴阴道假丝酵母菌病的检验方法比较

目的 比较外阴阴道假丝酵母菌病（VVC）的检验方法的差异.方法 采用生理盐水法、10%KOH法、革兰染色法和真菌培养法分别检测308份阴道分泌物,并对结果进行比较.结果 10%KOH法阳性187例（60.71%）,阴性121例 生理盐水法阳性180例（58.63%）,阴性128例 革兰染色法阳性198例（64.29%）,阳性110例 真菌培养法阳性207例（67.21%）,阴性101例.生理盐水法与10%KOH法、革兰染色法相比,阳性率差异有统计学意义 但与真菌培养法相比,阳性率差异没有统计学意义.10%KOH法与革兰染色法阳性率差异有统计学意义,但与真菌培养法相比,差异无统计学意义.革兰染色法与真菌培养法的阳性率差异亦无统计学意义.生理盐水法敏感度58.44%,10%KOH法敏感度60.71%,革兰染色法敏感度64.29%,3种方法的特异度均为100%.结论 生理盐水法与真菌培养法检验结果差异有统计学意义,10%KOH法、革兰染色法与真菌培养法检验结果差异无统计学意义.生理盐水法、10%KOH法、革兰染色法诊断敏感度依次升高.

Comparison of laboratory methods detecting vulvovaginal candidiasis

Objective To compare the differences of four laboratory methods of vulvovaginal candidiasis detection. Methods Three hundred and eight vagina secretions were tested by wet-mount and KOH preparations and Gram's stain and culture. Results There was a significant difference among wet-mount with KOH preparations and Gram's stain and culture. There was a significant difference between KOH preparations with Gram's stain. The test results of wet-mount and culture had statistical difference, but the result of KOH preparations and culture did not show statistical significance. The result between Gram's stain and culture did not show statistical significance.Conclusion The sensitivity of microscopic examination by wet-mount is higher than that of KOH test and Gram's stain.