乌拉尔甘草TIFY基因家族鉴定及调控分析＊

目的 为了探索乌拉尔甘草（Glycyrrhiza uralensis Fisch.）TIFY基因家族的特征并预测其功能,本研究在全基因组层面,应用生物信息学方法对乌拉尔甘草TIFY基因家族进行鉴定和分析。方法 在乌拉尔甘草全基因组数据基础上,利用NCBI、MEME及TBtools等工具,结合基因保守结构域,对乌拉尔甘草TIFY基因家族的序列特征、分类、系统发育和基因表达模式进行分析。结果 在乌拉尔甘草中鉴定到TIFY家族基因成员18个（GurTIFY1-GurTIFY18）,分别属于TIFY、JAZ、PPD、ZML四个亚家族,编码的核苷酸长度为444-1266 bp,氨基酸长度在147-421 aa之间,等电点为4.57-9.68,分子质量为16741.11-44426.5 Da,含有10个保守基序和4个保守结构域。基因表达结果表明GurTIFY基因中GurTIFY15、GurTIFY4、GurTIFY1、GurTIFY11等在乌拉尔甘草叶和根中的表达水平具有显著差异性。结论 本研究为深入理解TIFY基因响应非生物胁迫的调控机制和参与次生代谢物质累积的功能奠定基础,并为乌拉尔甘草优质品种选育提供科学参考。

Genome-wide Identification and Regulatory Analysis of the TIFY Gene Family in Glycyrrhiza uralensis

Objective To explore the characteristics of the Glycyrrhiza uralensis TIFY gene family and predict its function by using biological information to identify and analyze G. uralensis TIFY gene family from the whole genome level in G. uralensis.Methods Using the existing genomic data of G. uralensis, the GurTIFY genes were identified through bioinformatics analysis tools such as NCBI, MEME and TBtools etc, combined with the gene conserved domain, the sequence characteristics, taxonomy, phylogeny and gene expression patterns of the TIFY gene family were analyzed.Results The study identified 18 members of the TIFY family gene (GurTIFY1-GurTIFY18) in G. uralensis, which belong to four subfamilies: TIFY, JAZ, PPD and ZML. The GurTIFY were composed of 444-1266 bp nucleotides, amino acid length at 147-421 aa, isoelectric points of them were 4.57-9.68 and the molecular mass was 16741.11-44426.5 Da. By analyzing the gene structure, conservative motif could be known that the GurTIFY gene family contains 10 conservative motifs and 4 conservative domains. In addition, this study also analyzed the expression model of TIFY gene family members. The results showed that GurTIFY15, GurTIFY4, GurTIFY1, GurTIFY11 had significant differences in the leaf and roots of G. uralensis in all GurTIFY.Conclusion This study comprehensively analyzes the TIFY family in G. uralensis. The results of the study have laid the foundation for the in-depth understanding of the regulatory mechanism of TIFY gene response non-biodarity and the formation of the accumulation of secondary metabolism. This study will provide valuable reference for gene function study of the TIFY family genes in G. uralensis and G. uralensis breeding.