Real Time-PCR HBV-DNA Analysis: Significance and First Experience in Armed Forces |
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| Authors: | GS Chopra PK Gupta AC Anand PP Varma V Nair Ramji Rai |
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| Affiliation: | 1. Surgery Clinic and Transplantation Center, University Hospital in Martin and Jessenius Medical Faculty of the Comenius University, Martin, Slovak Republic;2. Transplant Department, L. Pasteur''s University Hospital, Košice, Slovak Republic;3. Department of Transplant Nephrology, II. Internal Clinic of Slovak Medical University, F.D. Roosevelt''s Faculty Hospital, Banská Bystrica, Slovak Republic;4. I. Internal Clinic, University Hospital Martin and Jessenius Medical Faculty of the Comenius University, Martin, Slovak Republic;5. Department of Urology and Renal Transplantation Center, University Hospital Bratislava and Medical Faculty of the Comenius University, Bratislava, Slovak Republic;6. Jessenius Medical Faculty of the Comenius University, Martin, Slovak Republic;1. University of Thessaly, School of Health Sciences, Department of Biochemistry & Biotechnology, Microbiology-Virology Laboratory, Larissa, Greece;2. General Hospital of Athens, Clinical Biochemistry Dpt, Athens, Greece;3. Research Centre of Oncology and Experimental Surgery, Anticancer Oncology Hospital of Athens “St Savvas”, Athens, Greece;1. Allergy Unit “D. Kalogeromitros”, Attikon University Hospital, University of Athens, Athens, Greece;2. Allergy Department, 2nd Pediatric Clinic, University of Athens, Athens, Greece;3. Department of Hematology, Attikon University Hospital, University of Athens, Athens, Greece;1. Institute of Functional Material Chemistry, Faculty of Chemistry, Northeast Normal University, Changchun 130024, Jilin, People''s Republic of China;2. Fundamental Department, Chinese People''s Armed Police Force Academy, Langfang 065000, People''s Republic of China |
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| Abstract: | ![]()
BackgroundHBV DNA quantitation is used extensively world wide for the diagnosis and monitoring of treatment of Hepatitis B virus (HBV) infection. However, it has still to be popular in India. The aim of this study was to quantitate HBV – DNA by Real time – PCR method in Hepatitis B and in immuno-compromised patients, to compare the results with HBeAg detection and to monitor the response to therapy of chronic Hepatitis B patients to antivirals.MethodsNinety one serum samples of Hepatitis group of patients (all HBsAg positive), 41 samples from immuno-compromised patients (all HBsAg negative) and 49 patients of Chronic Hepatitis B group (all HBsAg positive) were the subjects of this first ever study in Armed Forces. Twenty serum samples from healthy volunteers and non-hepatitis B patients served as negative controls. The amplification detection was carried out in a Rotor-Gene 2000-sequence detectorResultsAmongst Hepatitis B group, 33% (30/91) of the samples were positive for HBV-DNA and 26% (24/91) of samples were positive for HBeAg. In the immuno-compromised group of patients 14.6% (6/11) of samples were positive for HIV-DNA and 9.7% (4/41) were positive for HBeAg. Of the Chronic Hepatitis B patients on treatment, all (100%) were positive by HBV-DNA, whereas 29/49 (59.2%) were positive by HBeAg before treatment. After treatment with antivirals, 06/49 (12.2%) were positive by both tests and 11/49 (22.5%) were positive only by HBV-DNA. 32/49 (65.3%) patients became negative serologically after therapy.ConclusionHBeAg status did not necessarily reflect HBV-DNA level in the serum, as 10/91 (11%) in the Hepatitis B group, 2/41 (4.9%) in the immuno compromised group and 20/49 (40.8%) patients in the Chronic Hepatitis B group were positive for HBV-DNA but negative for HBeAg. HBV-DNA was not found to be positive amongst any of the negative controls. Real time – PCR is a sensitive and reproducible assay for HBV-DNA quantitation and may be started in Armed Forces referral centers in the near future.Key Words: Real time – PCR, Chronic Hepatitis B, HBV – DNA, Antivirals |
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| Keywords: | Real time – PCR Chronic Hepatitis B HBV – DNA Antivirals |
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