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| 实时荧光定量PCR和甲基化特异性PCR检测结直肠癌APC、MLH1基因甲基化状态 | |
| 引用本文： | 潘程宇,曹俊,季兴,张丽莉,郭慧敏,邹晓平. 实时荧光定量PCR和甲基化特异性PCR检测结直肠癌APC、MLH1基因甲基化状态[J]. 胃肠病学, 2011, 16(2): 77-81. DOI: 10.3969/j.issn.1008-7125.2011.02.004 |
| 作者姓名： | 潘程宇 曹俊 季兴 张丽莉 郭慧敏 邹晓平 |
| 作者单位： | 1. 南京医科大学鼓楼临床医学院消化科,210008 2. 南京大学医学院附属鼓楼医院消化科 3. 南京医科大学附属南京儿童医院药剂科 |
| 基金项目： | 江苏省医学重点人才项目资助 |
| 摘 要： | 背景：启动子区甲基化致肿瘤抑制基因失活在结直肠癌的发生、发展中起重要作用,检测肿瘤相关基因的甲基化状态,可能为寻找新的结直肠癌诊断、预后相关标记物提供依据.目的：比较实时荧光定量PCR（FO-PCR）与甲基化特异性PCR（MSP）检测基因甲基化状态的差异.方法：以FQ-PCR检测66例结直肠癌组织和20例癌旁组织中与结直肠癌的发生、发展相关的抑癌基因APC和错配修复基因MLH1的甲基化状态,同时以MSP检测结直肠癌组织中APC基因的甲基化状态.结果：根据FQ-PCR结果,结直肠癌组织中APC、MLH1基因甲基化阳性率显著高于癌旁组织（48.5%和54.5%对0%和0%,P=0.000）.FQ-PCR和MSP可检出的甲基化阳性对照DNA最低浓度分别为0.015 ng/μl和1.5 ng/μl,两者对APC基因甲基化状态的检测结果差异有统计学意义（P＜0.05）.结论：在DNA甲基化的检测手段中,FQ-PCR的敏感度优于MSP. |
| 关 键 词： | 结直肠肿瘤 DNA甲基化 基因，APC 基因，MLH1 甲基化特异性PCR 实时荧光定量PCR |
Detection of APC and MLH1 Gene Hypermethylation with Real-time Fluorescent Quantitative PCR and Methylation-specific PCR in Colorectal Cancer |
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| PAN Chengyu,CAO Jun,JI Xing,ZHANG Lili,GUO Huimin,ZOU Xiaoping. Detection of APC and MLH1 Gene Hypermethylation with Real-time Fluorescent Quantitative PCR and Methylation-specific PCR in Colorectal Cancer[J]. Chinese Journal of Gastroenterology, 2011, 16(2): 77-81. DOI: 10.3969/j.issn.1008-7125.2011.02.004 | |
| Authors: | PAN Chengyu CAO Jun JI Xing ZHANG Lili GUO Huimin ZOU Xiaoping |
| Affiliation: | PAN Chengyu,CAO Jun,JI Xing,ZHANG Lili,GUO Huimin,ZOU Xiaoping.Department of Gastroenterology,The Drum Tower Clinical Medical College of Nanjing Medical University,Nanjing(210008) |
| Abstract: | Background: Inactivation of tumor suppressor genes by promotor methylation plays an important role in the development and progression of colorectal cancer.Detection of hypermethylation of tumorigenesis-related genes might provide promising biomarkers for the diagnosis and prognosis of colorectal cancer.Aims: To compare the value of real-time fluorescent quantitative PCR(FQ-PCR) and methylation-specific PCR(MSP) for detecting the gene methylation.Methods: Hypermethylations of tumor suppressor gene APC and mi... |
| Keywords: | Colorectal Neoplasms DNA Methylation Genes APC MLH1 Methylation-Specific PCR Real-Time Fluorescent Quantitative PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |