原位杂交检测对曲霉菌属鼻窦炎诊断价值的探讨

目的探讨原位杂交技术在真菌性鼻窦炎病原学诊断方面的应用价值。方法应用针对曲霉菌属18SrRNA序列互补的18S-1寡核苷酸探针，经地高辛标记对37例真菌性鼻窦炎患者术后的石蜡包埋标本进行原位杂交检测。结果37例真菌性鼻窦炎标本中28例曲霉菌属原位杂交呈阳性反应，作为对照的白色念珠菌感染组织杂交结果为阴性。HE染色和六胺银染色可疑曲霉菌感染分别为21例和23例。结论真菌性鼻窦炎真菌培养阴性或无法进行真菌培养时，原位杂交是一种敏感、特异和快速检测组织内真菌病原菌的有效方法。

Diagnostic value for detection of aspergillus ribosomal RNA in fungal rhinosinusitis with hybridization in situ

OBJECTIVE: To develop a reliable, rapid assay for detecting pathogenic aspergillus species in fungal sinusitis. METHODS: Thirty-seven formalin-fixed and paraffin-embedded surgical tissue specimens from patients with fungal sinusitis were used in the present study. The aspergillus specific oligonucleotide probe was designed, commercially synthesized, and digoxigenin-labeled. Twenty-three-base oligonucleotides was selected that was complementry to 18S ribosomal RNA sequences (18S-1 probe) for detecting medically important aspergillus species. RESULTS: In situ hybridization for aspergillus rRNA was positive in 28 cases with the 18S-1 probe. Compared with HE (21) and methenamine-silver stain (23). CONCLUSION: In situ hybridization provides rapid and accurate identification for fungal organism in tissues, and may be useful if cultures are negative or have not performed.