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| 高效液相色谱法测定血及腹腔组织中丝裂霉素浓度 | |
| 引用本文： | 汤政,寇小格,杨留中,吴洁清,苗站会,张英鸽. 高效液相色谱法测定血及腹腔组织中丝裂霉素浓度[J]. 新乡医学院学报, 2007, 24(6): 567-570 |
| 作者姓名： | 汤政 寇小格 杨留中 吴洁清 苗站会 张英鸽 |
| 作者单位： | 新乡医学院法医病理学教研室; 新乡医学院第一附属医院肿瘤科; |
| 摘 要： | 目的应用反相高效液相色谱方法(HPLC)测定血及腹腔组织中丝裂霉素C(MMC)含量。方法血浆和组织液均用乙酸乙酯提取,利用反相高效液相色谱法测定组织中MMC。结果MMC组织浓度线性范围为80~8 000 ng.mL-1(r=0.996 94),方法回收率为84%~96%。日内日间精密度(RSD%)为1.45%~14.10%,最低检测浓度为5 ng.mL-1,高于现有其他方法。结论本方法简便、准确、灵敏,可用于科研和临床工作中血及组织中MMC浓度的快速检测。 |
| 关 键 词： | 高效液相色谱法 丝裂霉素C 乙酸乙酯 |
| 文章编号： | 1004-7239（2007）06-0567-04 |
| 收稿时间： | 2007-05-14 |
| 修稿时间： | 2007-05-14 |
Determination of mitomycin C in plasma and celiac tissue by high performance liquid chromatography method |
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| TANG Zheng,KOU Xiao-ge,YANG Liu-zhong,et al. Determination of mitomycin C in plasma and celiac tissue by high performance liquid chromatography method[J]. Journal of Xinxiang Medical College, 2007, 24(6): 567-570 | |
| Authors: | TANG Zheng KOU Xiao-ge YANG Liu-zhong et al |
| Affiliation: | TANG Zheng1,KOU Xiao-ge2,YANG Liu-zhong2,et al |
| Abstract: | Objective To determined the contents of mitomycin C(MMC) in plasma and celiac tissue by reversed-phase high performance liquid chromatography(RPHPLC) method.Methods Plasma and tissue suspension were extracted with ethyl acetate.MMC in plasma and celiac tissue was determined by RPHPLC.Results The linear range of MMC in tissue was from 80 ng·mL-1 to 8 000 ng·mL-1(r=0.996 94).The recovery was 84%～96%,The relative standard deviation within a day and between days was 1.45%～14.10 %.The minimum of quantitation was 5 ng·mL-1.Conclusion This method is sensitive and simple and accurate,and could be used for determining the concentration of MMC in blood and tissue in clinic and scientific research. |
| Keywords: | high performance liquid chromatography mitomycin C ethyl acetate |
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