| KGF-transfected cells can stimulate growth and proliferation of human cultured keratinocytes in vitro |
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| 引用本文： | Kopp J,Stark GB,Kulmburg p. KGF-transfected cells can stimulate growth and proliferation of human cultured keratinocytes in vitro[J]. 第二军医大学学报, 1998, 19(Z1): 74-76 |
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| 作者姓名： | Kopp J Stark GB Kulmburg p |
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| 作者单位： | Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai,200433,Department of Plastic and Hand Surgery, University Medical Center,Freiburg,Germany,From Department of Burn, Changhai Hospital, Second Military Medical University, Shanghai, 200433 |
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| 摘 要： | Objective: To establish two stably KGF-transfected, immortalized cell lines. Methods: HaCaT-keratinocytes and KMST-6-fibroblasts were transfected by liposome mediated gene transfer. Transfection effectivity, gene integration and configuration of the transgenic protein were investigated by ELISA, DANN-PCR and β-Gal-staining. Results: Most effective GF producing clones were tested by a colorimetric XTT-test. Conclusion: This is a significant acceleration of cell proliferation and mitosis of human keratinocytes in an Air Liquid Interface (ALI) test system.
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| 关 键 词： | keratinocyte growth factor gene transfection keratinocyte |
| 修稿时间： | 1998-06-01 |
KGF-transfected cells can stimulate growth and proliferation of human cultured keratinocytes in vitro |
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| Kopp J,Stark GB,Kulmburg p,Jiao XY,Dai FP,Chen YL. KGF-transfected cells can stimulate growth and proliferation of human cultured keratinocytes in vitro[J]. Former Academic Journal of Second Military Medical University, 1998, 19(Z1): 74-76 |
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| Authors: | Kopp J Stark GB Kulmburg p Jiao XY Dai FP Chen YL |
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| Abstract: | Objective: To establish two stably KGF-transfected, immortalized cell lines. Methods: HaCaT-keratinocytes and KMST-6-fibroblasts were transfected by liposome mediated gene transfer. Transfection effectivity, gene integration and configuration of the transgenic protein were investigated by ELISA, DANN-PCR and β-Gal-staining. Results: Most effective GF producing clones were tested by a colorimetric XTT-test. Conclusion: This is a significant acceleration of cell proliferation and mitosis of human keratinocytes in an Air Liquid Interface (ALI) test system. |
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| Keywords: | keratinocyte growth factor gene transfection keratinocyte |
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