帕罗西汀对束缚应激与额叶皮质相关性的影响研究

目的：观察束缚应激对大鼠额叶皮质的影响,探讨帕罗西汀在防治束缚应激引起焦虑的作用机制。方法：复制大鼠束缚应激模型,分为束缚应激组、治疗组和保护组,另设对照组（不束缚应激）,每组均n=10。观察大鼠行为学改变、额叶皮质c-fos基因表达、5-羟色胺（5-HT）和血浆皮质酮含量的变化,观察帕罗西汀干预的影响。结果：与对照组比较,束缚应激组大鼠额叶皮质的c-fos表达显著增加,5-HT表达明显减少,血浆皮质酮含量明显升高;与束缚应激组比较,治疗组和保护组血浆皮质酮含量和额叶皮质c-fos表达明显减少,5-HT表达明显增加。结论：额叶皮质参与了束缚应激反应;帕罗西汀抑制额叶皮质的c-fos基因表达、减少血浆皮质酮含量和增加额叶皮质5-HT含量可能是其抗焦虑作用机制之一。

Experimental Study of Restraint Stress Associated with the Frontal Cortex and the Impacts of Paroxetine on the Stress

To observe the effects of restraint stress on the frontal cortex of rats, and investigate the paroxetine’s mechanism of stress-induced anxiety. Methods： The rat models of restraint stress were established, which were divided into restraint stress group, treatment group, protection group, and control group. The rats’ behavioral changes, frontal cortex c-fos gene expression and the changes of leves of 5-HT and the plasma corticosterone, including the therapeutic effect of paroxetine, were observed. Results： Compared with the control group, the c-fos expression of frontal cortex of the rats in the restraint stress group was significantly increased, the 5-HT expression was significantly decreased, and the plasma corticosterone was significantly increased. Compared with the restraint stress group, the plasma corticosterone levels and the c-fos expression in frontal cortex in the treatment group and protection group were decreased, and the 5-HT expression was increased significantly. Conclusion： Frontal cortex involved in restraint stress. Paroxetine can inhibit the frontal cortex c-fos gene expression, reduce the levels of plasma corticosterone and increase the 5-HT content in frontal cortex. This may be one of the mechanisms of its anti-anxiety.