| 药物洗脱法模拟阿片类药物戒断细胞模型的建立 |
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| 引用本文： | 马浩,李斐,吴宁,李锦. 药物洗脱法模拟阿片类药物戒断细胞模型的建立[J]. 国外医学(药学分册), 2011, 0(4): 317-321 |
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| 作者姓名： | 马浩 李斐 吴宁 李锦 |
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| 作者单位： | Institute of Pharmacology and Toxicology;Academy of Military Medical Sciences; |
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| 基金项目： | 国家自然科学基金资助项目(30801394;30930040) |
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| 摘 要： | 目的应用药物急性洗脱法建立阿片类药物非纳洛酮催促戒断细胞模型。方法以稳定表达μ阿片受体（Mu opioid receptor,MOR）的CHO细胞CHO-MOR为研究对象,阿片类药物（D-Ala2,N-甲基-Phe,Gly-ol）-脑啡肽（DAMGO）及吗啡预处理细胞不同时间,然后采用药物洗脱法将药物去除,运用cAMP均相时间分辨荧光（HTRF）技术检测MOR下游信号通路第二信使分子cAMP的变化,以cAMP超射反映细胞水平阿片戒断。结果 DAMGO预处理细胞7.5 min、15min、30 min、1 h、2 h、3 h、4 h后,洗脱药物,使细胞脱离药物环境,cAMP含量随药物暴露时间延长逐渐升高,预处理3 h及4 h后,cAMP含量与对照组相比显著升高,即出现cAMP超射现象。吗啡预处理细胞1 h,胞内cAMP含量变化不明显,而预处理24 h后,将药物洗脱,cAMP含量与对照组相比显著升高。其cAMP超射值与应用纳洛酮催促戒断超射值无显著差异。结论药物洗脱法诱发的cAMP超射能够在细胞模型上较好的模拟阿片类药物戒断反应。
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| 关 键 词： | 药物洗脱 阿片类药物 戒断 细胞模型 |
Establishment of drug-eluting simulated opioids withdrawal cell model |
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| MA Hao,LI Fei,WU Ning,LI Jin. Establishment of drug-eluting simulated opioids withdrawal cell model[J]. Foreign Medical Sciences(Section of Pharmarcy), 2011, 0(4): 317-321 |
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| Authors: | MA Hao LI Fei WU Ning LI Jin |
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| Affiliation: | MA Hao,LI Fei,WU Ning,LI Jin(Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China) |
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| Abstract: | Objective To establish a kind of cell model of drug-eluting simulated opioids withdrawal,which distinguished from naloxone-precipitated withdrawal.Methods CHO-mu opioid receptor(MOR) cells which stablely express MOR were pretreated with(D-Ala2,N-Me-Phe,Gly-ol)-enkephalin(DAMGO) and morphine,then drugs were removed by drug-eluting.Using cAMP HTRF analysis detects the concentration of the second messenger cAMP in the down-stream pathways of MOR.The withdrawal index was cAMP overshooting.Results CHO-MOR cells ... |
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| Keywords: | drug-eluting opioids withdrawal cell model |
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