4NQO诱导小鼠舌癌前病变模型的研究

目的 使用4NQO诱导并建立C57BL/6J小鼠舌癌前病变模型.方法 46只6～8周龄C57BL/6J小鼠,随机取16只作为阴性对照,不做任何处理,分别于8、12、16周末处死.实验组30只饮用50μg/ml 4NQO水溶液,分别于8、12、16周末处死.处死前2小时腹腔注射BrdU (50mg/kg),肉眼及组织病...

A mouse model of tongue precancerous lesions induced by 4-nitroquinoline-1-oxide

Objective To establish an animal model of tongue precancerous lesions by using the carcinogen 4-nitroquinoline-1-oxide(4NQO).Methods Thirty C57BL/6J mice were divided into 3 groups,and given 50μg/ml 4NQO as drinking water for 8,12,16 weeks.Sixteen mice served as controls.The mice were sacrificed on weeks 8,12 and 16.All the mice were injected with 5-bromodeoxyuridine(BrdU) before they were sacrificed.Macroscopic and histopathological observations were performed on the samples of the tongue.Bromodeoxyuridine staining of the tissue was examined by immunohistochemistry.Results Macroscopic observation showed that white patches appeared in the tongue of the groups after 8-16 weeks of treatment by 4NQO.The numbers of mild and moderate dysplasia in the 4NQO-treated groups were 0.20±0.45,1.60±1.14,2.60±0.82 respectively.The number of BrdU-labeled cells was more in the 4NQO-treated groups than in the controls.Conclusion A mouse model of tongue precancerous lesions can be established by 50μg/ml 4NQO as drinking water.