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| 高速逆流色谱法分离小花黄堇中延胡索乙素和原阿片碱 | |
| 引用本文： | 吴东晶,吴建国,吴锦忠.高速逆流色谱法分离小花黄堇中延胡索乙素和原阿片碱[J].海峡药学,2011,23(10):63-65. |
| 作者姓名： | 吴东晶 吴建国 吴锦忠 |
| 作者单位： | 1. 福建中医药大学药学院 福州350108 2. 福建中医药大学中西医结合研究院 福州350108 3. 福建中医药大学药学院 福州350108;福建中医药大学中西医结合研究院 福州350108 |
| 摘 要： | 目的高速逆流色谱法分离纯化小花黄堇中延胡索乙素和原阿片碱。方法采用高速逆流色谱法进行分离，以正已烷．乙酸乙酯．甲醇．水（8：8：12：8，V／V）为溶剂系统．上相为固定相，下相为流动相，转速：800r·min一，流速：2mL·min－1，检测波长：280nm。结果从小花黄蔓总生物碱提取物中分离得到延胡索乙素和原阿片碱。纯度分别可达99．2％和99．6％。结论利用高速逆流色谱法可高效分离和纯化小花黄堇中延胡索乙素和原阿片碱。 |
| 关 键 词： | 高速逆流色谱 小花黄堇 延胡索乙素 原阿片碱 |
Preparative isolation of dl-tedrahypalmatine and protopine from Corydalis racemosa(Thunb.)Pers.by high-speed counter-current chromatography |
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| WU Dong-jing,WU Jian-guo,WU Jin-zhong.Preparative isolation of dl-tedrahypalmatine and protopine from Corydalis racemosa(Thunb.)Pers.by high-speed counter-current chromatography[J].Strait Pharmaceutical Journal,2011,23(10):63-65. | |
| Authors: | WU Dong-jing WU Jian-guo WU Jin-zhong |
| Institution: | WU Dong-jing,WU Jian-guo,WU Jin-zhong(Fujian university of traditional Chinese medicine,Fuzhou 350108,China) |
| Abstract: | OBJECTIVE To separate dl-tedrahypalmatine and protopine from Corydalis racemosa(Thunb.) Pers.by high-speed counter-current chromatography(HSCCC).METHODS Using high-speed counter-current chromatography,solvent systems was composed of n-hexane-ethylacetate-methanol-water(8∶8∶12∶8,v/v),and its upper phase was used as the stationary phase and its lower phase as the mobile phase,separation was performed at the rotating speed of 800r·min-1,flow rate of 2.0mL·min-1 and detection wavelength of 280nm.RESULTS Dl-tedrahypalmatine and protopine from Corydalis racemosa(Thunb.) Pers.were isolated with the purity of 99.2% and 99.6%.CONCLUSION Dl-tedrahypalmatine and protopine from Corydalis racemosa(Thunb.)Pers.could be separated and purified by HSCCC with high-efficiency. |
| Keywords: | High-speed counter-current chromatography Corydalis racemosa(Thunb ) Pers Dl-Tedrahypalmatine Protopine |
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