| 阿奇霉素对斑马鱼心脏毒性的初步研究 |
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| 引用本文： | 崔国祯,;周鹤峰,;陈焕娴,;李铭源.阿奇霉素对斑马鱼心脏毒性的初步研究[J].遵义医学院学报,2014(5):495-498. |
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| 作者姓名： | 崔国祯 ;周鹤峰 ;陈焕娴 ;李铭源 |
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| 作者单位： | [1]遵义医学院珠海校区生物工程系,广东珠海519041; [2]澳门大学中华医药研究院中药质量研究国家重点实验室,澳门999078 |
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| 基金项目： | 国家自然科学基金资助项目（NO：81328025）; 澳门科技发展基金项目（NO：014/2011/A1） |
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| 摘 要： | 目的建立阿奇霉素诱导斑马鱼幼鱼心脏毒性的模型,为后续抗菌类药物心脏毒性的机理研究奠定基础。方法以受精后2 d发育正常的斑马鱼胚胎作为模型,以不同浓度的阿奇霉素处理斑马鱼胚胎3 d后,观察斑马鱼胚胎心脏形态并计算其心率和心搏量以评价斑马鱼胚胎心脏的功能。结果 0.3和1 mm浓度的阿奇霉素作用3 d后,可见斑马鱼胎心明显毒性。与正常对照组比较,模型组斑马鱼胎心肿大,心率减慢和心搏量减小。结论阿奇霉素对斑马鱼胎心有明显毒性作用,且与阿奇霉素的浓度正相关,该模型可以用于后续抗生素类药物引起的心脏毒性的机理研究。
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| 关 键 词： | 阿奇霉素 心脏毒性 斑马鱼 |
Pilot study on cardiotoxicity induced by azithromycin in zebrafish embryos |
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| Institution: | Cui Guozhen, Zhou Hefeng , Chen Huanxian , Li Mingyuen (1. Department of Bioengineering, Zhuhai Campus of Zunyi Medical University, Zhuhai Guangdong 519041, China; 2. State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sci- ences, University of Macao, Macao 999078. China) |
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| Abstract: | Objective To investigate the physiological and pathological effects of cardiac toxicity induced by azithromycin in zebrafish and its underlying mechanisms.Methods Zebrafish embryos at 2 day post fertilization（ dpf） were treated with azithromycin for 3 days,and then the zebrafish hearts were evaluated for changes in morphology,heart rate and stroke volume by live imaging.Results Zebrafish exposed to 0.3-1 mM azithromycin resulted in enlarged heart and pericardial edema accompanied by significant decrease in the heart rate as well as stroke volume.Conclusion Zebrafish at early stage treated with various concentrations of azithromycin displayed cardiotoxicity.This model could be used for further investigation on the underlying mechanism of cardiotoxicity induced by azithromycin. |
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| Keywords: | azithromycin cardiotoxicity Zebrafish embryos |
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