小鼠胚胎干细胞植入大鼠脑内存活和增殖

【目的】观察小鼠胚胎干细胞 (ES细胞 )植入大鼠的隔区和海马内后存活和增殖的情况。【方法】以SD大鼠为宿主 ,将BrdU标记的ES细胞植入宿主的隔区和海马内 ,移植后l、2、3、4和 8周后取脑 ,冰冻切片 ,进行尼氏、BrdU、PCNA免疫组织化学染色 ,观察ES细胞存活和增殖的情况。【结果】ES细胞移植入大鼠隔区和海马内后 ,从第l周到第 3周 ,移植物呈团块生长 ,第 4周移植物较第 3周缩小 ,第 8周未发现移植物 ;BrdU和PCNA(核增殖抗原 )免疫染色结果阳性。【结论】小鼠ES细胞移植入大鼠隔区和海马内后 ,可以存活增殖 4周左右

Survival and Proliferation of Mouse Embryonic Stem Cells after Transplantation into the Adult Rat Brain

To observe the survival and proliferation of embryonic stem cells (ES cells) of mice after transplantation into the adult rat brain. BrdU marked ES BALB/C cells were implanted into the septum and hippocampus of adult rat brain and PCNA and BrdU were used to detect the implanted donor cells in the host brain In addition, survival and proliferation of ES cells were observed in the rat brain. From week 1 to week 3 after transplantation, the ES cells appeared either inside the septum or inside the hippocampus as a roundish mass Compared with that at week 3,the grafts volume began to decrease at week 4. Both the BrdU marked cells and the PCNA positive cells appeared inside the mass. [Conclusion] Transplanted ES cells can survive and proliferate for about 4 weeks in the septum and hippocampus of the rat brain after transplantation.