白英水提物对胃癌SGC-7901细胞凋亡与基因bcl-xl／bid表达的影响

目的:观察白英水提物对胃癌SGC-7901细胞凋亡及基因bcl-xl、bid表达的影响。方法:常规法制备白英水提物,实验分正常对照组、白英处理组。白英处理组加入白英水提物终浓度分别为12.5mg/mL、25mg/mL和50mg/mL。流式细胞仪检测细胞凋亡,半定量RT-PCR和免疫化学分析细胞中bcl-xl、bid基因表达。结果:与正常对照组比较,白英处理组SGC-7901细胞增殖有显著性下降,细胞凋亡显著性增多(P<0.05);基因bcl-xl mRNA和Bcl-xl蛋白表达降低、bidmR-NA和Bid蛋白表达升高(P<0.05),并随白英浓度增加而加强。结论:白英水提物能诱导人胃癌SGC-7901细胞凋亡和抑制增殖,且有一定的量效关系,其作用机制可能与调控bcl-xl、bid基因表达有关。

Influence of Solanum lyratum Thunb extract on apoptosis and the expression of bcl-xl/bid genes in human stomach cancer SGC-7901 cells

AIM:To investigate the effects of Solanum lyratum Thunb Extract(SLT) on apoptosis and the expression of bcl-xl,bid genes in human stomach cancer SGC-7901 cells.METHODS:Dried whole herbs of Solanum lyratum Thunb were extracted by boilingdistilled water.Human stomach cancer SGC-7901 cells were randomly divided into the control group and SLT-treated groups(12.5 mg/mL,25 mg/mL,50 mg/mL),cell apoptosis rate wase determined by flow cytometry.Expression of bcl-xl and bid mRNA were detected by semi-quantitive RT-PCR.Expression level of Bcl-xl and Bid proteins were detected by two-step immunhistochemical staining.RESULTS:Compared with control group,the proliferation inhibitory rate and apoptosis rate increased obviously(P<0.05).The expression of bcl-xl mRNA and Bcl-xl protein obviously decreased respectively(P<0.05).The expression of bid mRNA and Bid protein obviously increased respectively(P<0.05).CONCLUSION:SLT can induce apoptosis and inhibit proliferation of the Human stomach cancer SGC-7901 cells,and display effect in a dose-dependent manner by regulating expression of bcl-xl and bid genes.