前胡丙素对两肾两夹肾性高血压大鼠心脏重构及心功能的影响

目的研究前胡丙素长期灌胃给药对两肾两夹肾性高血压大鼠心脏、心功能及左室顺应性的影响。方法制作两肾两夹肾性高血压大鼠模型,前胡丙素20 mg.kg-1.d-1,连续灌胃给药9 wk,用离体大鼠工作心脏灌流法检测心功能及左室顺应性,W oessner等报道的方法测定心肌组织羟脯氨酸含量。结果与假手术对照组相比,肾性高血压左室肥厚(LVH)组大鼠CF/HWW和CO/HWW降低29.2%和38.5%,LVSP、-dp/dtm ax分别降低22.8%和43.2%;LV-EDP和T值增高172.9%和187.1%,LVEDP-V曲线左移上抬,左室肌胶原含量增加38.9%。前胡丙素组CF/HWW和CO/HWW比LVH组分别增加23.5%和37.2%,LVSP值和-dp/dtm ax负值较LVH组增加16.0%和42.7%,LVEDP和T值降低38.9%和39.0%,左室LVEDP-V曲线接近正常组水平,左室肌胶原含量降低18.9%,其作用与硝苯地平相似。结论前胡丙素可明显改善两肾两夹肾性高血压大鼠心脏舒张功能,改善心肌顺应性,同时也可改善心脏收缩功能,其作用可能是通过扩张冠状动脉,改善心肌缺血,降低心肌胶原含量而实现。

Effect of Praeruptorin C on cardiac remodelling, cardiac function in two-kidney two-clip renovascular hypertensive rats

Aim To study the effect of Praeruptorin C(Pra-C) on cardiovascular remodeling,cardiac function and left ventricular diastolic compliance by long-term treatment to two-kidney two-clip(2K2C) renovascular hypertensive rats(RHRs).Methods 2K2C operation was made to SD rats to establish hypertension and left heart hypertrophy(LVH) model,Pra-C 20 mg·kg~(-1)·d~(-1) was given ig for 9 weeks from the 4th week after 2K2C operation.The isolated working hearts and Langendorff′s hearts were made to study the effect of Pra-C on hemodynamics and myocardial compliance,and myocardial hydroxyproline content was measured with Woessner′s method to evaluate cardiac remodelling.Result Compared to sham-operated group,CF/HWW,CO/HWW and LVSP,-dp/dt_(max)(absolute value) were reduced by 29.2%,38.5%,22.8 %,43.2 %respectively,whereas LVEDP,T value and left ventricle collagen(represent by hydroxyproline) content increased by 172.9%,187.1%,38.9%,the left ventricular diastolic pressure-volume(P-V) curve was moved to left and up-word direction in RHR untreated group.Pra-C treated for 9 weeks leadto LVSP and-dp/dt_(max)(absolute val-(ue)) increased(16.0%,)(42.7%,)while LVEDP and T value and left ventricle collagen content decreased 38.9%,39.0% and 18.9% than those in RHR untreated group,and the LVEDP-V curve were shifted to the right and in the up-ward direction.Conclusion These results indicate that Pra-C improved the cardiac relaxation and contractility in 2K2C RHRs,which may be related with increasing the coronary flow and cardic output,decreasing the collagen content in the left ventricular myocardium.