复方茵柏颗粒对四氯化碳肝损伤模型大鼠氧化应激的影响

目的:研究复方茵柏颗粒对四氯化碳(CCl4)肝损伤模型大鼠氧化应激的影响。方法:48只SD大鼠随机分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、复方茵柏合剂(8.65 g/kg)组与复方茵柏颗粒高、中、低剂量(8.64、4.32、2.16 g/kg)组。灌胃给药,每天1次,连续3周。末次给药2 h后一次性腹腔注射40%CCl4的玉米油以复制大鼠急性肝损伤模型。测定大鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和乳酸脱氢酶(LDH)的活性,总胆红素(TBIL)、直接胆红素(DBIL)的含量;酶联免疫吸附(ELISA)法检测大鼠肝组织匀浆中丙二醛(MDA)和还原型谷胱甘肽(GSH)含量;并对大鼠肝组织作病理学检测。结果:与正常对照组比较,模型组大鼠血清ALT、AST、LDH活性显著增强,TBIL、DBIL含量显著增加,MDA含量显著增加,GSH活性显著减弱(P<0.01);与模型组比较,复方茵柏颗粒高、中、低剂量组大鼠血清ALT、AST、LDH活性显著减弱,TBIL、DBIL含量显著减少,MDA含量显著减少,GSH活性显著增强(P<0.01或P<0.05)。正常对照组大鼠肝小叶结构清晰,肝细胞索排列规则,肝细胞结构及形态正常,核大而圆,居中,核膜清晰;模型组大鼠大部分肝细胞明显水肿,气球样变,肝细胞索排列紊乱,肝小叶内中央静脉和汇管区出现弥漫性的炎细胞浸润;而复方茵柏颗粒高、中、低剂量组大鼠大部分肝细胞结构完整,排列整齐,肝细胞水肿、气球样变及炎细胞浸润均明显减轻。结论:复方茵柏颗粒对CCl4所致的大鼠急性肝损伤有一定的保护作用,其机制可能与其清除自由基、抑制脂质过氧化有关。

Effects of Compound Yinbai Granules on Oxidative Stress in Rats with Liver Injury Induced by Carbon Tet- rachloride

OBJECTIVE： To explore the effects of Compound yinbai granules on oxidative stress in rats with liver injury induced by carbon tetrachloride （CCl4）. METHODS： 48 SD rats were randomly divided into normal control group （constant volume of normal saline）, model group （constant volume of normal saline）, Compound yinbai mixture group （8.65 g/kg） and Compound yinbai granules high-dose, medium-dose and low-dose groups （8.64 g/kg, 4.32 g/kg, 2.16 g/kg）. They were given medicines intra- gastrically once a day for consecutive 3 weeks. Acute liver injury model was induced by intraperitoneal injection of maize oil containing 40% CCl4 2h after last administration. The activity of ALT, AST and LDH and the contents of TBIL and DBIL in serum were determined. ELISA was used to detect the contents of MDA and GSH; pathological test of liver was conducted. RESULTS： Compared with normal control group, the activities of ALT, AST and LDH, the contents of TBIL, DBIL and MDA were increased significantly in model group, while the activity of GSH was decreased significantly（P〈0.01）. Compared with model group, the ac- tivities of ALT, AST and LDH, the contents of TBIL, DBIL and MDA were decreased significantly in Compound yinbai granules high-dose, medium-dose and low-dose groups, while the activity of GSH was increased significantly（P〈0.01 or P〈0.05）. Clear structure of hepatic lobule, regular arranged hepatic cell cords, normal structure and morphology of hepatic cell, great round liver nuclei, centrally located and Clear caryotheca were observed in normal group; obvious swelling and ballooning degeneration of liv- er cell, disordered hepatic cell cords and diffuse inflammatory cell infiltration were observed in model group. The liver cells of rats were complete in structure and arranged, and edema, ballooning degeneration and inflammatory cell infiltration were relieved signif- icantly in Compound yinbai granules high-dose, medium-dose and low-dose groups. CONCLUSIONS：Compound yinbai granules have protection effect on acute hepatic injury in rats induced by carbon tetrachloride, and the mechanism is probably related to the scavenging of free radical and inhibition of lipid peroxidation.