| 正交试验优选野菊花中总黄酮的提取工艺 |
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| 引用本文： | 孔燕芳,周艳梅,闫艳仓,段晓颖.正交试验优选野菊花中总黄酮的提取工艺[J].中国药房,2013(43):4068-4070. |
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| 作者姓名： | 孔燕芳 周艳梅 闫艳仓 段晓颖 |
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| 作者单位： | [1]深圳健安医药有限公司,广东深圳518027 [2]河南中医学院第一附属医院,郑州450000 |
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| 摘 要： | 目的：优选野菊花中总黄酮的提取工艺。方法：以乙醇质量分数、加醇倍数、提取时间为考察因素，以野菊花总黄酮含量为评价指标，采用正交试验优选提取工艺。结果：最佳提取工艺为加12倍量50％乙醇，回流2次，每次1h。结论：所选工艺合理、可行，可用于野菊花中总黄酮的提取。
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| 关 键 词： | 野菊花 总黄酮 正交试验 紫外-可见分光光度法 |
Optimization of Extraction Technology of Total Flavonoids from Chrysanthemum indicum |
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| KONG Yan-fang,ZHOU Yan-mei,YAN Yan-cang,DUAN Xiao-ying.Optimization of Extraction Technology of Total Flavonoids from Chrysanthemum indicum[J].China Pharmacy,2013(43):4068-4070. |
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| Authors: | KONG Yan-fang ZHOU Yan-mei YAN Yan-cang DUAN Xiao-ying |
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| Institution: | 1.Shenzhen Jianan Pharmaceutical Co., Ltd., Shenzhen 518027, China; 2.The First Affiliated Hospital of Henan College of TCM, Zhengzhou 450000, China) |
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| Abstract: | OBJECTIVE： To optimize the extraction technology of total flavonoids from Chrysanthemum indicum. METHODS ： The extraction technology was optimized by orthogonal experiment with ethanol concentration, amount of ethanol, extraction time as factors using the content of total flavonoids from C. indicum as index. RESULTS： The optimal condition was as follows： adding 12 folds of 50% ethanol, reflux-extracting for 2 times, 1 h each time. CONCLUSIONS： The selected technology is reasonable and practicable, and it can be used for the extraction of total flavonoids from C. indicum. |
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| Keywords: | Chrysanthemum indicum Total flavonoids Orthogonal experiment UV-Visible spectrophotometry |
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