马齿苋总黄酮的提取工艺研究

目的:研究马齿苋总黄酮的提取工艺。方法:采用正交设计法及聚酰胺吸附分离法,以提取物中总黄酮含量为指标,考察马齿苋的提取工艺;并应用分光光度法测定提取物中总黄酮含量。结果:马齿苋的最佳提取工艺为每次加40倍的70%乙醇溶液,提取2次,每次提取60 min,所得提取液中的总黄酮含量最高;并应用聚酰胺吸附分离其中的总黄酮,以芦丁为标准品,以510 nm为检测波长,用UV法检测马齿苋总黄酮在0.007-0.0764 mg/ml范围内,浓度与吸光度呈良好的线性关系,相关系数R2=0.9991,该法所得提取物中总黄酮的相对含量最高为6.936%。结论:应用醇提、聚酰胺吸附分离法提取马齿苋中的总黄酮,方法简便、准确,为鉴定马齿苋的质量提供了一种可行的方法。

Study on the Best Extraction Technique of Total Flavonoids in Purslane

Objective： To study the optimum extraction technique of total flavonoids in Purslane.Methods： To select the optimum extraction technique for Purslane,Total flavonoids extracted with orthogonal design and by polycaprolactam adsorption method.Total flavonoids in Purslane were determined using UV spectrophotometry.Results： The optimum process of alcohol-decoction was to add 40 times amount of 70% alcohol,decocting for 2 times,60 minutes each time.the lever of total flavo-noids in hyperin was the highest.The contrast sample was rutin for ployamide-UV spectrophotometry.The optimum determination wavelength was 510nm.the result showed that a good linearity relationship（correlation coefficient：0.9991）between the degree of absorbance and the concentration of analyte was obtained,within the range of 0.007-0.0764mg/ml.the lever of total flavonoids in extracting agent was the highest using polycaprolactam,It＇s 6.936%.Conclusion： To extract flavonoids from Purslane by using alcohol-dococting and polycaprolactam,the method is convenient and exact.