去卵巢裸鼠人乳腺癌移植模型的建立

目的:采用雌激素皮贴刺激的方法,建立去卵巢(模拟妇女绝经后)裸鼠人乳腺癌移植模型,并探讨其部分生物学特性.方法:16只裸鼠切除卵巢后,背部给予雌激素皮贴,再接种雌激素受体阳性(ER )的MCF-7人乳腺癌细胞株,观察肿瘤块生长情况,30天后处死荷瘤裸鼠,切除肿块作病理切片及免疫组化.结果:接种后第4天即在接种部位可见结节,4周后肿瘤块平均表面积为(10.0±2.1)mm2,肿瘤移植成功率(成瘤率)为75%(12/16).病理学检查为浸润性导管癌,免疫组化雌激素受体表达呈阳性.结论:该方法采用了外部给予雌激素皮贴,刺激肿瘤的生长,成瘤率较高,且操作简便,成功的建立了去卵巢(绝经后)裸鼠人乳腺癌移植模型,经实验证明肿瘤可保持人乳腺癌生物学特性,为研究妇女绝经后雌激素依赖性乳腺肿瘤的发生机制提供了重要的科研工具.

Establishment of human breast carcinoma model in ovariectomized nude mice

Objective:To establish a model of human breast carcinoma in ovariectomized nude mice and study their biological features. Methods:MCF-7 cells(estrogen-receptor positive,ER+) were implanted into the flank of ovariectomized nude mice. The tumor was stimulated by estradiol E2 transdermal patches. Tumor size and body weight were monitored weekly during the study. The tumor-bearing mice were killed on day 30 and tumor masses were collected for analysis. Tumor tissues were pathologically examined by the light microscope. The ER protein was detected by immunohistochemistry. Results:The tumor tubercles could be formed on day 4. The tumor-take rate was 75%(12/16),and the mean tumor surface areas was(10.0 ± 2.1)mm2. All of the specimens were infiltrating ductal carcinoma,and ER expression was positive. Conclusion:Human breast carcinoma model was established successfully in ovariectomized nude mice,which reserved certain biological features of human breast carcinoma,and it was convenient to use transdermal patches,therefore it might be an available model for further research on breast carcinoma.