黄芩甙对HBsAg和HBeAg的体外抑制作用

目的：观察黄芩甙(Baicalin,Bai)对2．2．15细胞分泌HBsAg和HBeAg的影响，探讨黄芩甙的体外抗HBV作用。方法：通过四甲基偶氮唑盐(MTT)法观察黄芩甙对2．2．15细胞的影响，采用ELlSA法检测分析黄芩甙对2．2．15细胞分泌m6Ag、HBeAg的抑制作用。结果：黄芩甙在2．2．15细胞上半数毒性浓度大于15mg／ml，在该浓度下对HBsAg、HBeAg的抑制率分别达100％、88％以上，在黄芩甙浓度为0．94～15mg／ml时，对2．2．15细胞分泌HBsAg、HBeAg的抑制率高于70％，治疗指数分别为65．22和21．74。结论：黄芩甙有显著的体外抑制HBV作用，属低毒有效药物，具有潜在的抗HBV开发前景。

Inhibitory Effects of Baicalin on HBsAg and HBeAg in Vitro

Objective: To evaluate the effect of baicalin on excreting HBsAg and HBeAg of 2.2.15 cells. Methods: The toxicity of baicalin on 2.2.15 cell was detected by means of MTT. The inhibitory effects of baicalin on 2.2.15 cell excreting HBsAg and HBeAg were evaluated with method of ELISA. Results: The half toxic concentration of baicalin on 2.2.15 cell was higher than 15mg/ml, under this concentration, the inhibitory rate on HBsAg and HBeAg were as high as 100% and 88% respectively. The inhibitory concentration of excreting HBsAg and HBeAg on 2.2.15 cell of baicalin were between 0.94 and 15mg/ml, which was not lower than 70 %. The therapeutic indexes on HBsAg and HBeAg were 65.22 and 25.74 respectively. Conclusion: Baicalin can inhibit HBV significantly in vitro, it may be a kind of potential medicine with good effect and lower toxicity.