C-MYC基因在肝癌与肝硬化组织中扩增的实验研究

目的 研究C-MYC基因在肝癌及肝硬化组织中的扩增情况。方法 采用聚合酶链式反应(PCR)对32例肝癌及12例肝硬化组织中的C-MYC基因扩增情况进行研究，并通过激光密度扫描仪对琼脂糖凝胶EB染色底片进行积分光密度分析。结果 正常组织无C-MYC扩增，C-MYC与N-MYC L-MYC比值95％可信区间为0．1325-0．5632，肝硬化组织扩增阳性率为8．33％，肝癌组织扩增阳性率43．75％。结论 肝癌组织C-MYC扩增率高于正常组织和肝硬化组织，且C-MYC扩增者大多有淋巴结转移，组织分化差。检测C-MYC基因扩增情况可对肿瘤发生、发展及预后进行判断，并指导临床基因治疗。

Research on C - MYC oncogene amplification in the tissue of liver cancer and liver cirrhosis

Objective To study the amplification of C-MYC gene in 32 cases with liver cancer and 12 cases with liver cirrhosis.Methods C-MYC gene were detected by means of Polymerase Chain Reaction(PCR),and the results in films staining with EB were analyzed by integraling density.Results The amplification of C-MYC gene was not found in 32 normal control,and the average density of C-MYC/N-MYC+L-MYC was 0.1325~0.5632.The positive rate of C-MYC amplication in 12 liver cirrhosis was 8.33%,and that in 32 liver cancer was 43.75%.Conclusions Liver cancer's C-MYC positive rate is higher than that of liver cirrhosis and normal control,C-MYC gene was related with cell growth,differentiation and tumorous prognosis,the detecting C-MYC amplification was useful for gene therapy with antisense oligonucleotide.