| 用彗星试验研究饮用水中有机提取物对小鼠睾丸细胞DNA的损伤作用 |
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| 引用本文： | 王德东,钟嶷,朱伟,梁剑明,张潞,李文学,张全新,杨光宇,黄汝明,孙兰,傅雪松,施洁,周自严,毕华.用彗星试验研究饮用水中有机提取物对小鼠睾丸细胞DNA的损伤作用[J].热带医学杂志,2011,11(12):1375-1378,1480. |
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| 作者姓名： | 王德东 钟嶷 朱伟 梁剑明 张潞 李文学 张全新 杨光宇 黄汝明 孙兰 傅雪松 施洁 周自严 毕华 |
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| 作者单位： | 广州市疾病预防控制中心;广东药学院;广州军区疾病预防控制中心; |
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| 基金项目： | 广州市卫生局资助项目(2009-YB-120) |
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| 摘 要： | 目的通过彗星试验探讨饮用水两种不同消毒工艺所产生的非挥发性有机提取物对小鼠睾丸细胞DNA的损伤作用。方法设对照组,低、中和高剂量组共4个组,采用彗星试验检测NZ和XC水厂丰水期和枯水期水源水、自来水水样的非挥发性有机提取物对小鼠睾丸细胞DNA的损伤,并进行比较。结果各剂量组都出现了明显的拖尾现象,NZ与XC水厂的拖尾率比较差异无统计学意义。各剂量组的平均尾长明显高于对照组,NZ水厂低剂量组中,枯水期水源水平均尾长(19.3±2.7)μm高于自来水的(16.0±2.5)μm;高剂量组中,丰水期水源水高于自来水。水源水中,枯水期低剂量组低于丰水期、中剂量组高于丰水期;自来水中,枯水期中剂量组低于丰水期,高剂量组高于丰水期。XC水厂低剂量组中,丰水期的水源水平均尾长(15.5±1.0)μm低于自来水的(24.9±2.6)μm,中剂量组中,枯水期水源水高于自来水。水源水中,丰水期低剂量组低于枯水期,中剂量组和高剂量组丰水期高于枯水期;自来水中,丰水期中剂量组高于枯水期。结论 NZ和XC水厂的丰水期和枯水期有机提取物均含有导致小鼠睾丸细胞DNA损伤的物质;水源水与自来水、丰水期与枯水期表现为不同程度的DNA损伤。
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| 关 键 词： | 有机提取物 饮用水 氯化消毒 睾丸细胞 DNA损伤 彗星试验 |
Experimental study of the organic extracts of drinking water on DNA damage of testicle cells in mice by the comet assay |
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| WANG De-dong,ZHONG Yi,ZHU Wei,LIANG Jian-ming,ZHANG Lu,LI Wen-xue,ZHANG Quan-xin,YANG Guang-yu,HUANG Ru-ming,SUN Lan,FU Xue-song,SHI Jie,ZHOU Zi-yan,BI Hua.Experimental study of the organic extracts of drinking water on DNA damage of testicle cells in mice by the comet assay[J].Journal Of Tropical Medicine,2011,11(12):1375-1378,1480. |
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| Authors: | WANG De-dong ZHONG Yi ZHU Wei LIANG Jian-ming ZHANG Lu LI Wen-xue ZHANG Quan-xin YANG Guang-yu HUANG Ru-ming SUN Lan FU Xue-song SHI Jie ZHOU Zi-yan BI Hua |
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| Institution: | WANG De-dong1,ZHONG Yi1,ZHU Wei1,LIANG Jian-ming2,ZHANG Lu3,LI Wen-xue1,ZHANG Quan-xin1,YANG Guang-yu1,HUANG Ru-ming1,SUN Lan1,FU Xue-song1,SHI Jie1,ZHOU Zi-yan1,BI Hua1 (1.Guangzhou Center for Disease Control and Prevention,Guangdong,Guangzhou 510440,2.Guangdong Pharmaceutical University,Guangzhou 510310,3.Guangzhou Command for Disease Control and Prevention,Guangzhou 510507,China) |
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| Abstract: | Objective Compare the DNA damage effects on testicle cells in mice of the non-volatile chlorination by-products generated from two different drinking water disinfection processes. Methods The mice were randomly divided into four groups: negative group, low-dose group, middle-dose group and high-dose group. The testicular cell DNA damages by the organic extracts from the water samples from the water source of the NZ and XC manufactories during wet season and dry season, and also the tap water sample were det... |
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| Keywords: | organic extract drinking water chlorination disinfection testicular cells DNA damage single cell gel electrophoresis |
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