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| 实时荧光定量PCR技术诊断肺结核的meta分析 | |
| 引用本文： | 郑芬,吴奎海,孙静静,芮勇宇. 实时荧光定量PCR技术诊断肺结核的meta分析[J]. 中国热带医学, 2011, 11(11): 1315-1317 |
| 作者姓名： | 郑芬 吴奎海 孙静静 芮勇宇 |
| 作者单位： | 南方医科大学南方医院检验科,广东广州,510515 |
| 摘 要： | 目的系统评价实时荧光定量PCR技术对肺结核的诊断价值并作meta分析。方法检索Pubmed,EMBASE,BIOSIS,Web of Science以及维普、万方、中国知网、中国医学生物文献数据库。按照Cochrane协作网推荐的诊断试验纳入标准筛选文献,纳入的文献采用QUADAS进行质量评价,用MetaDisc 1.4进行meta分析。结果符合标准的共有21个研究,各研究间存在异质性,但不存在阈值效应。合并后的诊断优势比为105.32,敏感度为0.64,特异度为0.96,阳性似然比为19.79,阴性似然比为0.31。SROC曲线的AUC面积为0.9816,Q＊指数为0.9402。结论荧光定量PCR技术检测结核分枝杆菌有着较高的敏感性和很强的特异性,可作为临床肺结核诊断的重要辅助手段,但并不能完全取代痰涂片检查。 |
| 关 键 词： | 实时荧光定量PCR 肺结核 meta分析 |
Use of fluorescence quantitative PCR for the detection of Mycobacterium tuberculosis gene:A meta-analysis |
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| ZHENG Fen,WU Kui-hai,SUN Jing-jing,et al.. Use of fluorescence quantitative PCR for the detection of Mycobacterium tuberculosis gene:A meta-analysis[J]. China Tropical Medicine, 2011, 11(11): 1315-1317 | |
| Authors: | ZHENG Fen WU Kui-hai SUN Jing-jing et al. |
| Affiliation: | ZHENG Fen,WU Kui-hai,SUN Jing-jing,et al.(Department of Clinical Laboratory,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China) |
| Abstract: | Objective To make a systematic review and meta-analysis on the use of fluorescence quantitative PCR(FQ-PCR) assay for rapid detection of M.tuberculosis.Methods Pubmed,EMBASE,BIOSIS,VIP,WANFANG DATA,CNKI,CBM were searched for studies about FQ-PCR assay in the diagnosis of M.tuberculosis.The inclusion of qualified literatures was based on the criteria for diagnostic research recommended by the Cochrane Methods Group on Screening and Diagnostic Test.Quality of included trials was assessed by QUADAS(quality ass... |
| Keywords: | Fluorescence quantitative PCR Pulmonary tuberculosis Meta-analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |