FHIT基因微卫星变异与宫颈癌关系的分析

[目的]探讨FHIT基因微卫星变异与宫颈癌的关系。[方法]选取FHIT基因的两个微卫星多态标记,采用聚合酶链反应法（PCR）对50例宫颈癌及40例宫颈上皮内瘤样病变（CIN）组织进行杂合性丢失（LOH）和微卫星不稳定性（MI）的检测。[结果]在D3S1234位点上宫颈癌的LOH发生率为46%（23/50）,CIN的LOH发生率为40%（16/40）,宫颈癌与CIN的LOH发生率差异无统计学意义（P〉0.05）,宫颈癌的MI发生率为18%（9/50）,CIN的MI发生率为12.5%（5/40）,差异无统计学意义（P〉0.05）;在D3S4103位点上宫颈癌的LOH发生率为40%（20/50）,CIN的LOH发生率为37.5%（15/40）,差异无统计学意义（P〉0.05）,宫颈癌的MI发生率为14%（7/50）,CIN的MI发生率为7.5%（3/40）,差异无统计学意义。但宫颈癌在两位点上LOH和MI发生频率均高于CIN,且高级别CIN发生率高于低级别CIN。[结论]FHIT基因的变异发生在宫颈癌变的晚期;FHIT基因的LOH与MI对于宫颈癌的筛查、早期诊断及判断预后可能具有临床实用价值。

Relationship between FHIT Gene Microsatellite Alterations and Cervical Carcinoma

[Purpose] To explore the relationship between FHIT gene microsatellite alterations and cervical carcinoma. [Methods] Loss of heterozygosity （LOH） and microsatellite instability（MI） at two sites of microsatellite polymorphism of FHIT gene were detected by polymerase chain reaction（PCR） technique in 50 cases with cervical carcinoma and 40 cases with cervical intraepithelial neoplasia（CIN）. [Results] At D3S1234,the LOH rate of cervical carcinoma was 46%（23/50） and the LOH rate of CIN was 40%（16/40）（P〉0.05）. The MI rate of cervical carcinoma was 18%（9/50） and the MI rate of CIN was 12.5%（5/40）（P〉0.05）. At D3S4103,the LOH rate of cervical carcinoma was 40%（20/50） and the LOH rates of CIN was 37.5%（15/40）（P〉0.05）.The MI rate of cervical carcinoma was 14%（7/50） and the MI rate of CIN was 7.5%（3/40）（P〉0.05）. The rates of LOH and MI in cervical carcinoma were higher than those in CIN. The rates of LOH and MI in high grade CIN were higher than those in low grade CIN. [Conclusion] The FHIT gene alteration is a relatively late event in cervical carcinoma. The detection of LOH and MI of FHIT gene might be helpful to the early diagnosis and the screening for cervical carcinoma. It might also be useful for predicting the prognosis for cervical carcinoma.