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| JC-1单标法检测精索静脉曲张患者精子线粒体膜电位的研究 | |
| 引用本文： | 胡毅,夏欣一,潘连军,吕年青,吴永明,周鑫,商学军,崔英霞,黄宇烽. JC-1单标法检测精索静脉曲张患者精子线粒体膜电位的研究[J]. 中华男科学杂志, 2009, 15(9): 792-795 |
| 作者姓名： | 胡毅 夏欣一 潘连军 吕年青 吴永明 周鑫 商学军 崔英霞 黄宇烽 |
| 作者单位： | 1. 南京大学医学院临床学院,南京军区南京总医院男科,江苏,南京,210002 2. 南京医科大学附属南京市妇幼保健院泌尿男科,江苏,南京,210004 3. 江苏省计划生育科研所,江苏,南京,210019 4. 广西医科大学第一附属医院人类精子库,广西,南宁,530021 |
| 基金项目： | 江苏省科技厅生殖健康研究技术服务平台项目(BM2008151)~~ |
| 摘 要： | 目的:检测精索静脉曲张患者精子线粒体膜电位并探讨其临床意义。方法:将67例精索静脉曲张患者分为VC1组(精索静脉曲张1度,n=26)、VC2组(精索静脉曲张2度,n=21)和VC3组(精索静脉曲张3度,n=20),以正常生育男性为正常对照组(n=29)。通过计算机辅助精液分析系统进行精液常规分析。精液标本洗涤处理后用荧光染料JC-1染色后上流式细胞仪分析,检测精子线粒体膜电位(JC-1+%)。结果:VC1、VC2、VC3组精子线粒体膜电位[(56.29±16.32)%,P<0.05;(45.04±13.21)%,P<0.01;(31.63±12.91)%,P<0.01]均显著低于正常对照组[(76.21±13.96)%]。96例标本中,JC-1+%与(a+b)级精子百分率呈显著正相关(r=0.693,P=0.000)。结论:精索静脉曲张可引起精子线粒体膜电位降低,可能是导致男性不育的重要原因之一。 |
| 关 键 词： | 精索静脉曲张 精子 线粒体膜电位 流式细胞术 |
Evaluation of Sperm Mitochondrial Membrane Potential in Varicocele Patients Using JC-1 Fluorescent Staining |
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| HU Yi,XIA Xin-yi,PAN Lian-jun,L Nian-qing,WU Yong-ming,ZHOU Xin,SHANG Xue-jun,CUI Ying-xia,HUANG Yu-feng. Evaluation of Sperm Mitochondrial Membrane Potential in Varicocele Patients Using JC-1 Fluorescent Staining[J]. National journal of andrology, 2009, 15(9): 792-795 | |
| Authors: | HU Yi XIA Xin-yi PAN Lian-jun L Nian-qing WU Yong-ming ZHOU Xin SHANG Xue-jun CUI Ying-xia HUANG Yu-feng |
| Affiliation: | HU Yi,XIA Xin-yi,PAN Lian-jun,L(U) Nian-qing,WU Yong-ming,ZHOU Xin,SHANG Xue-jun,CUI Ying-xia,HUANG Yu-feng |
| Abstract: | Objective:To detect sperm mitochondrial membrane potential (MMP) of varicocele patients and investigate its clinical significance. Methods: Sixty-seven varicocele patients were divided into a VC1 (grade 1,n=26),a VC2 (grade 2,n=21) and a VC3 group (grade 3,n=20). And 29 normal fertile volunteers were included in a control group ( n=29). Conventional semen analyses were performed by computer-assisted semen analysis (CASA). Semen samples were washed,followed by JC-1 staining to evaluate the sperm MMP (JC-1+%)... |
| Keywords: | varicocele sperm mitochondrial membrane potential flow cytometry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |