肝硬化大鼠脊髓内一氧化氮合酶阳性神经元的分布

背景肝硬化时可对脊髓等身体其他组织器官产生严重的影响.目的应用四氯化碳损伤大鼠肝脏制备肝硬化大鼠模型,探讨肝硬化大鼠脊髓内一氧化氮合酶分布情况.设计完全随机对照实验.单位首都医科大学解剖教研室.材料实验于2002-03/2003-12在首都医科大学解剖教研室完成.20只Wistar雄性大鼠随机分为肝硬化组和正常组,每组10只.方法肝硬化组经四氯化碳损伤大鼠肝脏制备肝硬化大鼠模型,正常大鼠组不作任何处理.于模型制备后3个月,各组大鼠在麻醉状态下开胸,灌注,固定,取脊髓组织,制备切片,采用还原型烟酰胺腺嘌呤二核苷酸磷酸黄递酶组化法及Leica Q500IW图像分析系统对肝硬化大鼠脊髓一氧化氮合酶阳性细胞进行数量及灰度的测定.主要观察指标1]两组大鼠一氧化氮合酶阳性神经元灰度值.2]两组大鼠一氧化氮合酶阳性细胞分布情况.结果20只大鼠均进入结果分析.1]两组大鼠一氧化氮合酶阳性神经元灰度均为60(P＞0.05).2]在脊髓灰质区,一氧化氮合酶阳性细胞主要分布在中央管周围即脊髓板层第Ⅹ层和中间外侧核.还原型烟酰胺腺嘌呤二核苷酸磷酸黄递酶组化法呈色为强阳性,细胞形态多为三角形和梭形,胞核不着色,胞质色深.细胞中等大小,以25 μm为主.在脊髓颈、胸、腰各段灰质中间带一氧化氮合酶阳性细胞分布的数量基本相等,没有特异性变化.结论肝硬化大鼠与正常大鼠-氧化氮合酶在脊髓内的表达基本相同.一氧化氮在脊髓节段的分布特性可能说明肝硬化大鼠在低级交感神经的调控上与正常无差异.

Distribution of nitric oxide synthase positive neurons in spinal cord of rats with cirrhosis

BACKGROUND: Cirrhosis can cause severe effect on spinal cord or other tissue and organ sometimes.OBJECTIVE: To establish cirrhosis model with rat liver injured by tetrachloride so as to investigate the distribution of nitric oxide synthase (NOS)in spinal cord of rats with cirrhosis.DESIGN: Completely randomized controlled study.SETTING: Anatomy Department of Capital University of Medical Sciences.MATERIALS: The experiment was completed at the Anatomy Department of Capital University of Medical Sciences from March 2002 to December 2003. Totally 20 male Wistar rats were divided randomly into cirrhosis group and normal group with 10 in each group.METHODS: Cirrhosis model in cirrhosis group was established with rat liver injured by tetrachloride, but rats in normal group were not treated with any method. After 3 months, total rats in the two groups were perfused and fixed; meanwhile, tissue of spinal cord was taken out for section.Quantity and gray degree of NOS positive cell in spinal cord of rats with cirrhosis were measured with nicotinamide adenine dinucleotide hydrogen phosphate-diaphorase (NADHP-d) histochemical method and Leica Q500IW image analysis system.MAIN OUTCOME MEASURES: 1] Gray degree of NOS positive neurons of rats in the two groups; 2] Distribution of NOS positive cell of rats in the two groups.RESULTS: Twenty rats all entered the final analysis. 1] Gray degree of NOS positive neurons of rats in the two groups was 60 (P ＞ 0.05). 2] In the area of gray matter of spinal cord, NOS positive cells were mainly distributed over the circumference of central canal, i.e. the Ⅹ layer of spinal cord and intermediolateral nucleus. Color of NADHP-d was positive, and the cellular form was shaped in triangle and fusiform. Cellular nucleus was not colored but color of cytoplasm was deep. The size of cells was moderate mainly of 25 μm. NOS positive cells were generally distributed averagely over intermediate zone of gray matter in cervical, thoracic and lumbar spinal cord and had no specific changes.CONCLUSION: Expression of NOS in spinal cord of rats in the cirrhosis group and the normal group is probably identical. Distributive characteristics of nitrogen monoxide (NO) in spinal cord suggest that adjustment on low sympathetic nerve of rats with cirrhosis is not different from that of the normal.