| 电磁场促进骨髓间充质干细胞体外诱导分化时细胞增殖 |
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| 引用本文： | 朱淑霞,李永华,宋治远,罗向东,仝识非. 电磁场促进骨髓间充质干细胞体外诱导分化时细胞增殖[J]. 第三军医大学学报, 2008, 30(5): 421-423 |
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| 作者姓名： | 朱淑霞 李永华 宋治远 罗向东 仝识非 |
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| 作者单位： | 1. 第三军医大学西南医院心血管内科,重庆市介入心脏病学研究所,重庆400038
2. 第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆,400083 |
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| 摘 要： | 目的 观察脉冲电磁场(pulsed electromagnetic fields,PEMF)对5-氮胞苷(5-aza)诱导大鼠骨髓问充质干细胞(mesenehymal stem cells,MSCs)向心肌样细胞分化时细胞增殖的影响.方法 50 Hz脉冲低频电磁场刺激5-aza诱导后7 d后的MSCs,根据不同的感应强度分为A、B、C组,每组根据作用时间的不同分为4个亚组;设未暴露PEMFs的细胞为对照组.用MTT法测定细胞增殖变化,用流式细胞技术检测细胞周期.结果 0.5 mT和1 mT的感应强度下20～30 min细胞比对照组有显著性增殖,5 mT作用30～60 min以及1 mT作用60 min与对照组相比较呈显著性抑制.流式检测细胞周期显示0.5 mT和1 mT在20～30 min内可以引起细胞S G2%增高,各感应强度下作用60 min以及5 mT感应强度下作用20 min以上均引起S G2%的下降.结论 50 Hz脉冲低频电磁场0.5 mT和1 mT的感应强度下,作用20～30 min可通过引起诱导后的MSCs周期中S G2%增高而促进它的增殖.
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| 关 键 词： | 脉冲电磁场 骨髓间充质干细胞 增殖 |
| 文章编号： | 1000-5404（2008）05-0421-03 |
| 收稿时间： | 2007-11-30 |
| 修稿时间： | 2008-01-04 |
Pulsed electromagnetic fields improve proliferation of rat marrow mesenchymal stem cells induced by 5-azacytidine |
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| ZHU Shu-xia,LI Yong-hua,SONG Zhi-yuan,LUO Xiang-dong,TONG Shi-fei. Pulsed electromagnetic fields improve proliferation of rat marrow mesenchymal stem cells induced by 5-azacytidine[J]. Acta Academiae Medicinae Militaris Tertiae, 2008, 30(5): 421-423 |
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| Authors: | ZHU Shu-xia LI Yong-hua SONG Zhi-yuan LUO Xiang-dong TONG Shi-fei |
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| Affiliation: | 1Department of Cardiology;2State Key Laboratory of Trauma;Burns and Combined Injury;Institute of Burns;Southwest Hospital;Third Military Medical University;Chongqing 400038;China |
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| Abstract: | Objective To investigate the effects of pulsed electromagnetic fields on the proliferation of rat marrow mesenchymal stem cells induced by 5-azacytidine. Methods Rat marrow mesenchymal stem cells induced for 7 d with 5-azacytidine were exposed or unexposed to 50 Hz pulsed electromagnetic fields and then randomly divided into A, B, C groups and 1, 2, 3, 4 subgroups according to different inductance intensity and different exposed time. Unexposed under pulsed electromagnetic fields was control groups . Prolif... |
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| Keywords: | pulsed electromagnetic field mesenchymal stem cells proliferation |
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