赤雹根总皂苷对类风湿性关节炎大鼠血清细胞因子的影响

目的研究赤雹根总皂苷对类风湿性关节炎大鼠血清IL-1β、IL-6、TNF-α的影响。方法以弗氏完全佐剂诱导大鼠佐剂性关节炎,将造模成功50只大鼠随机分为模型组,赤雹根总皂苷160,80,40 mg/(kg·d)组和雷公藤12 mg/(kg·d)组,另取10只正常大鼠作正常对照。连续灌胃给药21 d。观测各组大鼠继发关节肿胀度和关节炎指数;给药后第22天,处死大鼠,用酶联免疫吸附法(ELISA)检测血清IL-1β、IL-6、TNF-α含量。结果赤雹根总皂苷能明显降低佐剂性关节炎大鼠继发侧的关节肿胀度和关节炎指数(P<0.01);与正常对照组相比,模型组大鼠血清IL-1β、IL-6、TNF-α含量明显升高(P<0.01);赤雹根总皂苷灌胃治疗后,各组大鼠血清IL-1β、IL-6、TNF-α含量较模型组明显降低(P<0.01)。结论赤雹根总皂苷对大鼠类风湿性关节炎有治疗作用,且该作用与抑制IL-1β、IL-6、TNF-α的合成有关。

Effect of the Total Saponins of the Thladiantha dubia on Roots Serum Cytokines in Rheumatoid Arthritis Rats

Objective To study the effect of the total saponins of the Thladiantha dubia roots on the levels of serum cytokines IL-1β,IL-6 and TNF-α in rheumatoid arthritis rats.Methods The Arthritis(AA) rat model was induced by adjuvant AA rats were divided into five groups:model group(distilled water),SRTD 160 mg·kg-1·d-1,80mg·kg-1·d-1,40mg·kg-1·d-1groups and Tripterygium12 mg·kg-1·d-1 group,gavage administration for 21 days.The joint swelling degree and arthritic index in the secondary side of rats was observed.The contents of cytokines IL-1β,IL-6,TNF-α were determined by ELISA.Results Joint swelling degree and arthritic index of AA rats in STTD groups were lower than those in model group(P<0.01).The content of serum cytokines IL-1β,IL-6,TNF-α of AA rats in STTD groups were decreased as compared to model group(P<0.01).Conclusion The SRTD has therapeutic effect on RA rats.The role is closely related to the inhibition on the synthesis of cytokines IL-1β、IL-6 and TNF-α.