胶束毛细管电泳法测定丹参及复方丹参片中迷迭香酸的含量

目的建立胶束毛细管电泳测定丹参及复方丹参片中迷迭香酸的含量。方法重力进样75 s,缓冲溶液为25 mmol/L硼砂-40 mmol/L十二烷基硫酸钠,pH值为9.85,电压为13 kV,检测波长为320 nm。结果在上述条件下,迷迭香酸(RA)的线性回归方程式为Y=18 117X+3 157.4(r=0.996 8),线性范围为2.5～100μg/ml,检出限达到0.25μg/ml,迷迭香酸的回收率为92%～105%,RSD为3.2%。结论该方法简单、准确,可用于丹参及复方丹参片中迷迭香酸的含量测定。

Determination of Rosmarinic Acid in Salvia miltiorrhiza and Compound Salvia Tablets by Micellar Electrokiectic Capillary Chromatography

Objective To develop a new method for determination of rosmarinic acid in Salvia miltiorrhiza and compound Salvia miltiorrhiza tablet by micellar electrokinetic capillary electrophoresis.Methods The sample was injected in 75 s,the buffer solution was composed of 25 mmol/L borax-40mmol/L SDS,pH was 9.85,the applied voltage was 13 kV and detection wavelength was set at 320 nm.Results Under the optimum conditions,linear equation of RA was y=18117x+3157.4,the correlation was 0.9968.The linear range was 2.5~100 μg/ml,the limit of determination was 0.25 μg/ml.The recovery was found in the range from 92% to 105%,with RSD of 3.2%.Conclusion The method is simple and accurate.It can be used to detect rosmarinic acid in salvia miltiorrhiza and compound salvia tablets.