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| Effect of NS398 on inducing apoptosis and down-regulating Bcl-2 protein in human osteosarcoma cell MG-63 line | |
| 引用本文： | Eryou Feng,Biao Gao,Renyun Xia. Effect of NS398 on inducing apoptosis and down-regulating Bcl-2 protein in human osteosarcoma cell MG-63 line[J]. 南京医科大学学报(自然科学版), 2005, 19(4): 199-202 |
| 作者姓名： | Eryou Feng Biao Gao Renyun Xia |
| 作者单位： | [1]Department of Orthopedics, TongJi Hospital,Tongji Medical College, Huazhong University of Science and Techology, Wuhan 430030, China; [2]Department of Orthopedics, Center Hospital of Xiaogan City, Xiaogan 432100,China. |
| 摘 要： | Objective： This study investigated the effect of NS398 on anti-proliferation and the mechanism of inducing apoptosis of human osteosarcoma cell MG-63 line in vitro. Methods： Growth suppression was detected by MTT method. Special morphological changes of apoptosis were observed by transmission electron microscopy （TEM） and DNA fragments electrophoresis. The apoptotic rates were quantified by flow cytometry （FCM）. And Bcl-2 protein level were detected by Western Blotting assay. Results： Different concentration NS398 inhibited the cell growth. Through TEM and DNA electrophoresis, the special morphological changes were observed after 24, 48 and 72 h treatment with NS398. The apoptotic rates of MG-63 cells treated with NS398 were respectively, significantly higher than that of the control group（ P 〈0.01 ）. Western blot assay showed that NS398 reduced Bcl-2 protein expression. Conclusion： NS398 suppressed the proliferation of human osteosarcoma cell MG-63 line and induced apoptosis. The mechanism may be associated with down-regulation expression level of bcl-2 protein. |
| 关 键 词： | NS398 Bcl-2蛋白 骨肉瘤 肿瘤细胞 MG-63 |
| 收稿时间： | 2005-01-18 |
Effect of NS398 on inducing apoptosis and down-regulating Bcl-2 protein in human osteosarcoma cell MG-63 line |
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| Eryou Feng,Biao Gao,Renyun Xia. Effect of NS398 on inducing apoptosis and down-regulating Bcl-2 protein in human osteosarcoma cell MG-63 line[J]. Acta Universitatis Medicinalis Nanjing, 2005, 19(4): 199-202 | |
| Authors: | Eryou Feng Biao Gao Renyun Xia |
| Abstract: | Objective: This study investigated the effect of NS398 on anti-proliferation and the mechanism of inducing apoptosis of human osteosarcoma cell MG-63 linein vitro. Methods: Growth suppression was detected by MTT method. Special morphological changes of apoptosis were observed by transmission electron microscopy (TEM) and DNA fragments electrophoresis. The apoptotic rates were quantified by flow cytometry (FCM). And Bcl-2 protein level were detected by Western Blotting assay. Results: Different concentration NS398 inhibited the cell growth. Through TEM and DNA electrophoresis, the special morphological changes were observed after 24, 48 and 72 h treatment with NS398. The apoptotic rates of MG-63 cells treated with NS398 were respectively, significantly higher than that of the control group(P<0.01). Western blot assay showed that NS398 reduced Bcl-2 protein expression. Conclusion: NS398 suppressed the proliferation of human osteosarcoma cell MG-63 line and induced apoptosis. The mechanism may be associated with down-regulation expression level of bcl-2 protein. |
| Keywords: | cycloosygenase-2 osteosarcoma MG-63 cell apoptosis |
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