人脐静脉内皮细胞的培养及鉴定

目的：摸索人脐静脉内皮细胞体外培养的方法和注意事项。方法：采用0．25％胰蛋白酶分离人脐静脉内皮细胞，含20％FBSDMEM养基培养，待细胞80％融合后，以O．25％胰蛋白酶消化传代，并以倒置显微镜观察内皮细胞的生长情况，以免疫组化方法对内皮细胞进行鉴定。结果：原代培养的细胞在接种4h后开始贴壁生长，3－5天后融合成单层，倒置显微镜下观察细胞呈铺路石状排列，免疫组化显示人第Ⅷ因子相关抗原阳性。结论：用胰蛋白酶消化脐静脉可获得内皮细胞且成活率较高，是一种可靠的方法。

Culture and Identification of the Human Umbilical Vein Endothelial Cell in Vitro

Objective ： To explore the culture and identification of the human umbilical vein endothelial cell （ HUVEC ） in vitro. Methods ： HUVECs were obtained from human umbilical vein by the technique of trypsinase digestion and cultivated in DMEM which containing 20% FBS; the passage was developed with 0.25% trypsinase when confluensing. The cells were identified under the microscope with immunohistochemical method. Results ： The primary cells began to attach and grow after being inoculated 4 hours, and grew to monolayer 3-5 days later. The antigens of human VIU factor were positive according to immunohistochemical method. Conclusion ： Trypsinase digestion is an effective way to cultivate the human umbilical vein endothelial cells in vitro.