| RP-HPLC法测定排石利胆颗粒中芍药苷含量 |
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| 引用本文： | 张颖,张起辉,邸子真,邹桂欣,尤献民. RP-HPLC法测定排石利胆颗粒中芍药苷含量[J]. 辽宁中医学院学报, 2011, 0(9): 197-198 |
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| 作者姓名： | 张颖 张起辉 邸子真 邹桂欣 尤献民 |
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| 作者单位： | [1]辽宁省中医药研究院,辽宁沈阳110034 [2]重庆大学化学化工学院,重庆400030 |
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| 摘 要： | 目的：建立排石利胆颗粒中芍药苷的含量测定方法。方法：采用RP-HPLC法测定含量,使用Hypersil C18（5μm,4.6mm×200mm）色谱柱,紫外检测波长230nm,乙腈-0.1%磷酸（13：87）为流动相,流速1.0mL/min。结果：通过方法学考察,芍药苷在0.08104～0.48624μg与峰面积呈良好的线性关系,r=0.9996,平均回收率96.2%（RSD=1.1%,n=9）。结论：RP-HPLC法可用于排石利胆颗粒中芍药苷的含量测定。
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| 关 键 词： | RP—HPLC 排石利胆颗粒 芍药苷 |
Determination of Paeoniflorin in Paishi Lidan Granule by RP-HPLC |
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| ZHANG Ying,ZHANG Qi-hui,DI Zi-zhen,ZOU Gui-xin,YOU Xian-min. Determination of Paeoniflorin in Paishi Lidan Granule by RP-HPLC[J]. Journal of Liaoning College of Traditional Chinese Medicine, 2011, 0(9): 197-198 |
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| Authors: | ZHANG Ying ZHANG Qi-hui DI Zi-zhen ZOU Gui-xin YOU Xian-min |
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| Affiliation: | 1. Liaoning Province Academy of Chinese Medicine, Shenyang 110034, Liaoning, China; 2.College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China ) |
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| Abstract: | Objective：To establish the determination method of the concent of paeoniflorin in Paishi Lidan granule by HPLC.Method：The column was Hpersil C18（5μm,4.6mm×200mm）,the detection wavelength was at 230nm,the mobile phase was the mixture of acetonitrile-0.1% phosphoric acid（13：87）,the flow rate is 1.0mL/min.Result：A good linear correlation of paeoniflorin was observed between 0.08104～0.48624μg,r =0.9996,the average recovery was 96.2% with RSD of 1.1%（n=9）.Conclusion：The method can be used as the quality control method of this preparation. |
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| Keywords: | RP-HPLC Paishi Lidan granule paeoniflorin |
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