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Generation of human monoclonal antibody-producing cell lines by Epstein-Barr virus (EBV)-transformation of B lymphocytes and somatic cell hybridization techniques
Authors:Hideyuki Ikematsu  Inna S. Goldfarb  Nagaradona Harindranath  Marion T. Kasaian  Paolo Casali
Affiliation:(1) Department of Pathology and Kaplan Cancer Center, New York University School of Medicine, MSB-599, 550 First Avenue, 10016 New York, New York;(2) Laboratory of Molecular Cancer Genetics, National Cancer Institute, National Institutes of Health, 20892 Bethesda, Maryland;(3) Laboratory of Oral Medicine, National Institutes of Health, 20892 Bethesda, Maryland
Abstract:
Summary The use of Epstein-Barr virus to immortalize human B cells and generate monoclonal antibody-producting B cell lines is described.
Keywords:Epstein-Barr virus  human monoclonal antibody
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