EB病毒感染与XRCC1-Arg399Gln基因多态性在鼻咽癌发生中的交互作用

目的评估XRCCl-Arg399 Gln单核苷酸多态性在鼻咽癌发病中的风险,研究XRCCl-Arg399 Gln单核苷酸多态性与EB病毒感染的交互作用。方法采用病例对照研究,收集90名经病理确诊的鼻咽癌患者作为病例组和75名在我院进行健康体检的病人作为对照,并按照性别和年龄1∶1配对,提取全血基因组DNA进行PCR扩增,对扩增产物再进行测序PCR反应,最后分析得出XRCC1 Arg399Gln的基因型。结果 EB病毒感染能增加鼻咽癌发病风险,VCA-IgA、EA-IgA和Rta-IgG抗体三项阳性时可以增加鼻咽癌发病风险,但单项VCA-IgA阳性是鼻咽癌发病的最强危险因素(OR=26.526,95%CI:11.190~62.884),单因素条件Logistic回归分析显示,与携带XRCC-1 Arg399Gln的AA基因型相比,携带XRCC-1 Arg399Gln的GG基因型可增加鼻咽癌发病风险(OR=4.3,95%CI:1.184~15.618);XRCC-1 Arg399Gln的GA基因型与VCAIgA存在交互作用(OR=20.755,95%CI:5.312~81.094)。结论 XRCC-1基因的Arg399Gln基因多态性能够增加患鼻咽癌的风险,且Arg399Gln的GA基因型与EB病毒感染具有一定的交互作用。

Associations and interactions between EB virus infection and XRCC1 Arg399Gln polymorphism in nasopharyngeal car-cinoma

Objective To investigate the correlations between XRCC1 Arg399Gln polymorphism and the risk of nasopharyn-geal carcinoma (NPC),and trace the mutual interactions between XRCC1 polymorphisms and the EB virus infection. Methods In the matched case-control study,90 NPC patients and 75 healthy controls were enrolled. Genome DNA were extracted from the whole blood and then amplified via PCR. The PCR products were further purified and sent for DNA sequencing to get the genotype of XRCC1 Codon399. At the same time,the S/CO values of VCA-IgA,EA-IgA and Rta-IgG were evaluated using ELISA,and the positive as well as the constituent ratios were assessed. Results VCA-IgA,EA-IgA and Rta-IgG positivewas regarded as a high risk factor in NPC occurrence,especially that single VCA-IgA positive seemed to be achieve the highest OR value of 26.526 (95%CI:11.190-62.884). Univairate logistic regression analysis showed that the OR value of patients with XRCC-1 Arg399Gln GG genotype was more higher that that of AA genotype;There was the mutual interactions between XRCC-1 Arg399Gln GA genotype and VCA-IgA(OR=20.755,95%CI:5.312-81.094). Conclusions Arg399Gln GG genotype was associated with the susceptibility of NPC;there were interactions between(XRCC1) Arg399Gln single nucleotide polymorphisms and EB virus infection in NPC.